PpYUC11, a strong candidate gene for the stony hard phenotype in peach (Prunus persica L. Batsch), participates in IAA biosynthesis during fruit ripening.
PpYUC11, a strong candidate gene for the stony hard phenotype in peach (Prunus persica L. Batsch), participates in IAA biosynthesis during fruit ripening.
复制标题
PpYUC11 是桃 (Prunus persica L. Batsch) 石硬表型的有力候选基因，参与果实成熟过程中的 IAA 生物合成。
DOI：
10.1093/jxb/erv400
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发表时间：
2015-12
影响因子：
6.9
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Pan L;Zeng W;Niu L;Lu Z;Liu H;Cui G;Zhu Y;Chu J;Li W;Fang W;Cai Z;Li G;Wang Z
PpYUC 11是控制桃石硬表型的一个强候选基因。高浓度的吲哚-3-乙酸（IAA）是跃变型乙烯生物合成所必需的，从而导致果实软化。相比之下，由于IAA浓度低，石质硬桃品种的果实不软化，产生很少的乙烯。为了研究桃成熟过程中IAA积累的调控[从S3阶段过渡到S4阶段III（跃变型）]，进行了数字基因表达（DGE）分析。比较了果肉软化桃‘金蜜3号’和果肉坚硬桃‘玉明’果实成熟过程中生长素稳态相关基因的表达模式。结果表明，生长素合成的关键基因YUCCA黄素单加氧酶基因（PpYUC 11，ppa 008176 m）与生长素积累和PpACS 1转录的差异表达谱一致：在果肉化桃中，其mRNA转录本在成熟后期增加，而在硬石桃中则低于检测限。此外，PpYUC 11内含子TC微卫星基因型与43个桃品种的果肉质地（正常或石硬）有较强的相关性，表明该位点可能是桃石硬表型的主要原因。这些发现支持了PpYUC 11可能在桃果实成熟过程中生长素生物合成中起重要作用的假设，并且是控制桃石硬表型的候选基因。
PpYUC11 is a strong candidate gene for the control of the stony hard phenotype in peach. High concentrations of indole-3-acetic acid (IAA) are required for climacteric ethylene biosynthesis to cause fruit softening in melting flesh peaches at the late ripening stage. By contrast, the fruits of stony hard peach cultivars do not soften and produce little ethylene due to the low IAA concentrations. To investigate the regulation of IAA accumulation during peach ripening [the transition from stage S3 to stage S4 III (climacteric)], a digital gene expression (DGE) analysis was performed. The expression patterns of auxin-homeostasis-related genes were compared in fruits of the melting flesh peach ‘Goldhoney 3’ and the stony hard flesh peach ‘Yumyeong’ during the ripening stage. It is revealed here that a YUCCA flavin mono-oxygenase gene (PpYUC11, ppa008176m), a key gene in auxin biosynthesis, displayed an identical differential expression profile to the profiles of IAA accumulation and PpACS1 transcription: the mRNA transcripts increased at the late ripening stage in melting flesh peaches but were below the limit of detection in mature fruits of stony hard peaches. In addition, the strong association between intron TC microsatellite genotypes of PpYUC11 and the flesh texture (normal or stony hard) is described in 43 peach varieties, indicating that this locus may be responsible for the stony hard phenotype in peach. These findings support the hypothesis that PpYUC11 may play an essential role in auxin biosynthesis during peach fruit ripening and is a candidate gene for the control of the stony hard phenotype in peach.