The diagnosis of bovine virus diarrhoea-mucosal disease in cattle.
The diagnosis of bovine virus diarrhoea-mucosal disease in cattle.
复制标题
牛病毒性腹泻粘膜病的诊断。
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
S. Edwards
中科院分区：
文献类型：
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作者：
S. Edwards
本文综述了牛病毒性腹泻和粘膜病的实验室诊断程序。对于血清学，重点放在病毒中和试验和酶免疫测定上。的技术和陷阱，可能会遇到在培养过程中的病毒分离的描述，特别提到使用免疫荧光和免疫过氧化物酶的方法检测非致细胞病变的病毒株。病毒抗原也可直接在临床或病理样品中检测。单克隆抗体有望在诊断试验中发挥越来越大的作用，而基因探针在病毒检测中的应用也有望在未来几年内实现。在诊断实验室检测结果的解释中，强调了在牛群疾病和育种史的背景下对其进行审查的重要性，并将其与当前对疾病发病机制和流行病学的理解联系起来。
Laboratory diagnostic procedures for bovine virus diarrhoea and mucosal disease are reviewed. For serology, emphasis is placed on the virus neutralisation test and enzyme immunoassays. The techniques and the pitfalls which may be encountered in culture procedures for isolation of the virus are described, with special reference to the use of immunofluorescence and immunoperoxidase methods for the detection of non-cytopathogenic virus strains. Viral antigen may also be detected directly in clinical or pathological samples. Monoclonal antibodies are expected to play an increasing role in diagnostic tests, and the application of gene probes for virus detection is anticipated in future years. In the interpretation of diagnostic laboratory test results, the importance is stressed of reviewing them in the context of the herd disease and breeding history, and relating them to current understanding of the pathogenesis and epidemiology of the disease.
牛病毒性腹泻病毒肺炎株互补DNA的分子克隆及其诊断应用。
DOI：
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发表时间：
1988
期刊：
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
影响因子：
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作者：
Brock,KV;Brian,DA;Rouse,BT;Potgieter,LN
通讯作者：
Potgieter,LN