Testis-specific lactate dehydrogenase (LDH-C4; Ldh3) in murine oocytes and preimplantation embryos
Testis-specific lactate dehydrogenase (LDH-C4; Ldh3) in murine oocytes and preimplantation embryos
复制标题
DOI：
10.2164/jandrol.05185
复制
发表时间：
2006-07-01
影响因子：
--
通讯作者：
Goldberg, Erwin
中科院分区：
文献类型：
--
作者：
Coonrod, Scott;Vitale, Alejandra;Goldberg, Erwin
LDH-C-4 (Ldh3) 是乳酸脱氢酶同工酶家族的成员，可催化糖酵解途径中的末端反应。在哺乳动物中，ldha、ldhb 和 Ldhc 3 个基因编码组装成具有催化活性的同源四聚体和异源四聚体的亚基。这些基因的差异表达决定了组织中乳酸脱氢酶（LDH）同工酶的组成，众所周知，A亚基在骨骼肌中占主导地位，B亚基在大脑和心脏中大量产生，其中Ldh2同工酶在卵母细胞中含量最丰富。 C肽首先在粗线期精母细胞中检测到，构成精子的初级LDH。最初，基于免疫化学、酶学和分子分析，Ldhc 基因（适用于小鼠细胞的术语为 Ldh3）被认为是睾丸特异性的。在这里，我们报告了在小鼠卵母细胞和早期胚胎中检测到这种同工酶。我们的结果表明 Ldh3 mRNA 在卵母细胞中转录，在受精卵中无法检测到。然而，LDH3 蛋白持续存在于胚胎发育的囊胚阶段，主要定位于卵母细胞、卵子、受精卵和胚胎卵裂球的皮质区域。
LDH-C-4 (Ldh3) is a member of the lactate dehydrogenase family of isozymes that catalyze the terminal reaction in the glycolytic pathway. In mammals, 3 genes, ldha, ldhb, and Ldhc, encode the subunits that assemble into catalytically active homo- and heterotetramers. Differential expression of these genes determines the lactate dehydrogenase (LDH) isozyme composition of tissues, and, as is well known, A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart, with the Ldh2 isozyme the most abundant form in oocytes. The C peptide can be detected first in pachytene spermatocytes and constitutes the primary LDH of spermatozoa. Originally the Ldhc gene (Ldh3 in terminology applied to murine cells) was considered to be testis specific on the basis of immunochemical, enzymatic, and molecular analyses. Here we report the detection of this isozyme in the murine oocyte and early embryo. Our results indicate that Ldh3 mRNA is transcribed in oocytes and cannot be detected in fertilized eggs. Ldh3 protein, however, persists to the blastocyst stage of embryonic development localizing mainly to the cortex region of oocytes, eggs, zygotes, and embryonic blastomeres.