Genome-wide identification of microRNAs responding to early stages of phosphate deficiency in maize
Genome-wide identification of microRNAs responding to early stages of phosphate deficiency in maize
复制标题
全基因组鉴定响应玉米早期磷酸盐缺乏的 microRNA
DOI：
10.1111/ppl.12409
复制
发表时间：
2016
影响因子：
6.4
通讯作者：
Gao Shibin
中科院分区：
文献类型：
--
作者：
Nie Zhi;Ren Zhiyong;Wang Libo;Su Shunzong;Wei Xuan;Zhang Xiao;Wu Ling;Liu Dan;Tang Haitao;Liu Hailan;Zhang Suzhi;Gao Shibin
磷（P）是参与许多生物化学反应的必需元素。在植物中，胁迫反应，如microRNA（miRNAs）的表达，被诱导以帮助它们适应低磷酸盐（Pi）浓度。在这项研究中，使用在低Pi浓度下生长的玉米幼苗的根和叶进行深度测序，以鉴定在Pi缺乏的早期阶段差异表达的miRNA。构建了8个小RNA文库，其中159个已知的miRNA代表32个miRNA家族和10个新的miRNA。miR 396家族的成员非常丰富。此外，鉴定了28种Pi响应性miRNA（27种已知的和1种新的），其中8种和7种分别在叶和根组织中显著表达。对Pi响应性miRNAs靶基因的分析表明，大多数靶基因作为转录因子参与根和叶的发育。通过定量真实的时间聚合酶链反应（qRT-PCR）确认所选Pi响应性miRNA和靶基因的表达谱。此外，我们讨论了这些miRNA的表达模式的差异在Pi饥饿的早期和后期阶段的意义。本研究提供了有关miRNAs在磷饥饿反应中的作用的有用信息，并将进一步加深我们对玉米磷稳态调控机制的理解。
Phosphorus (P) is an essential element involved in numerous biochemical reactions. In plants, stress responses, such as the expression of microRNAs (miRNAs), are induced to help them adapt to low phosphate (Pi) concentrations. In this study, deep sequencing was performed using the roots and leaves of maize seedlings grown under low Pi concentrations to identify miRNAs that are differentially expressed during the early stages of Pi deficiency. Eight small RNA libraries were constructed, and 159 known miRNAs representing 32 miRNA families and 10 novel miRNAs. Members of the miR396 family were extremely abundant. Further, 28 Pi‐responsive miRNAs were identified (27 known and 1 novel) of which 8 and 7 were significantly expressed exclusively in leaf and root tissues, respectively. The analysis of Pi‐responsive miRNAs target genes suggested that most target genes functioning as transcription factors were involved in root and leaf development. The expression profiles of selected Pi‐responsive miRNAs and target genes were confirmed by quantitative real‐time polymerase chain reaction (qRT‐PCR). Moreover, we discuss the significance of the differences in expression patterns of these miRNAs during the early and later stages of Pi starvation. This study provides useful information concerning the role of miRNAs in response to Pi starvation and will further our understanding of the mechanisms governing Pi homeostasis in maize.