Steady state and time-resolved fluorescence properties of metastatic and non-metastatic malignant cells from different species
Steady state and time-resolved fluorescence properties of metastatic and non-metastatic malignant cells from different species
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DOI：
10.1016/1011-1344(95)07178-4
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发表时间：
1995-12-01
影响因子：
5.4
通讯作者：
Blanchard, L
中科院分区：
文献类型：
--
作者：
Pradhan, A;Pal, P;Blanchard, L
用稳态荧光光谱和时间分辨荧光光谱研究了来自不同物种的未转移、转移和非致瘤细胞系的荧光。用310 nm和350 nm激发分别监测色氨酸和还原型烟酰胺腺嘌呤二核苷酸(NADH)的荧光。不同类型的细胞系之间存在细微而一致的差异，发现转移细胞系的色氨酸/NADH荧光强度比在未转移细胞系和正常细胞系中更高。不同细胞系中色氨酸残基的荧光衰减可以用三重指数动力学来描述，而NADH的荧光衰减主要可以用双指数动力学来描述，在某些情况下也可以用三重指数动力学来描述。色氨酸的平均荧光寿命为2.5-3.7 ns。转移细胞中NADH的平均寿命比非转移细胞低(大约三倍)，这一发现在不同来源的细胞系(大鼠或人)中是一致的。对每个物种的平均荧光寿命和每个细胞系的体积进行了荧光强度校正，结果表明，在恶性转移癌细胞中，色氨酸和NADH的浓度始终高于未转移细胞。
Steady state and time-resolved fluorescence spectroscopy were employed to study the fluorescence from non-metastatic, metastatic and non-tumorigenic cell lines from different species. Excitations at 310 nm and 350 nm were used to monitor tryptophan and reduced nicotinamide adenine dinucleotide (NADH) fluorescence respectively. Subtle and consistent differences were observed between different categories of cell lines, It was found that the tryptophan to NADH fluorescence intensity ratio is higher in metastatic cell lines than in non-metastatic and normal cell lines. The fluorescence decay of the tryptophan residue in different cell lines was best described by triple exponential kinetics, whereas the NADH fluorescence decay was best described by mainly double and, in some cases, triple exponential kinetics. The average fluorescence lifetimes for tryptophan were in the range 2.5-3.7 ns. The average lifetime of NADH was lower (by a factor of approximately three) in metastatic cells than in non-metastatic cells and this finding is consistent for cell lines from different origins (rat or human). Correcting the fluorescence intensity for the average fluorescence lifetime of each species and for the volume of each cell line, it was shown that the concentrations of tryptophan and NADH are consistently higher in malignant metastatic cancer cells than in non-metastatic cells.