KLK5 and KLK7, two members of the human tissue, kallikrein family, are differentially expressed in lung cancer
KLK5 and KLK7, two members of the human tissue, kallikrein family, are differentially expressed in lung cancer
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DOI：
10.1016/j.bbrc.2005.02.100
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发表时间：
2005-04-22
影响因子：
3.1
通讯作者：
Courty, Y
中科院分区：
文献类型：
--
作者：
Planque, C;de Monte, M;Courty, Y
新出现的数据表明，肌动蛋白家族（KLK）的丝氨酸蛋白酶与多种人类疾病有关，包括癌症；然而，kallikrein基因在肺癌中的表达从未被研究过。通过RT-PCR和Western blotting，我们证实了KLK5和KLK7及其各自的蛋白（hK5和hK7）在肿瘤和非肿瘤肺组织中的表达。然后通过实时RT-PCR分析56例非小细胞肺癌患者的定量基因表达。KLK5在鳞状细胞癌中的表达明显高于匹配的非恶性肺组织（P = 0.02），而KLK7在腺癌中的表达则降低（P = 0.003）。多因素分析显示，KLK5和KLK7在非恶性和恶性组织中的表达水平与临床参数（包括组织类型、转移状态和分级）之间存在不同的相关性。我们的研究结果为激肽激酶基因在激素非依赖性癌中的表达提供了新的见解。总之，我们的研究结果表明，KLK5和KLK7基因表达的变异性可能参与了肺肿瘤的发生，并对临床有帮助。(c) 2005爱思唯尔公司版权所有。
Emerging data indicate that serine proteases of the kallikrein family (KLK) are implicated in various human diseases, including carcinoma; however, kallikrein gene expression has never been investigated in lung cancer. Using RT-PCR and Western blotting, we demonstrated the expression of both KLK5 and KLK7, and their respective proteins (hK5 and hK7) in tumoral and nontumoral lung tissues. Quantitative gene expression was then analyzed in a cohort of 56 patients with non-small cell lung cancer by real-time RT-PCR. KLK5 expression is significantly more expressed in squamous cell carcinoma than in matched nonmalignant lung tissue (P = 0.02), whereas expression of KLK7 was decreased in adenocarcinoma (P = 0.003). Multivariate analysis revealed diverse correlations between the KLK5 and KLK7 expression levels in nonmalignant and malignant tissues, and clinical parameters, including histotype, metastatic status, and grade. Our findings provide new insight into kallikrein gene expression in hormone-independent carcinoma. Altogether, our results suggest that variability in KLK5 and KLK7 gene expression might be involved in lung tumorigenesis and useful for clinical purposes. (c) 2005 Elsevier Inc. All rights reserved.