ADRENOCORTICOTROPIN IS A SPECIFIC MITOGEN FOR MAMMALIAN MYOGENIC CELLS
ADRENOCORTICOTROPIN IS A SPECIFIC MITOGEN FOR MAMMALIAN MYOGENIC CELLS
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DOI：
10.1016/s0012-1606(89)80006-5
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发表时间：
1989-02-01
影响因子：
2.7
通讯作者：
MOLINARO, M
中科院分区：
文献类型：
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作者：
COSSU, G;CUSELLADEANGELIS, MG;MOLINARO, M
已发现源自阿片黑皮素原 (POMC) 的肽可刺激小鼠生肌细胞的增殖。在这些肽中，发现促肾上腺皮质激素(ACTH)和α-、β-和γ-黑素细胞刺激激素(MSH)具有活性，而阿片样肽则没有活性。在克隆密度方面，ACTH 和 MSH 均导致肌原性集落中每个克隆的平均细胞数增加三到四倍，但在成纤维细胞集落中则不然。在高细胞密度下，ACTH 和 MSH 导致生肌细胞的增殖增加三到四倍，这反映在骨骼肌球蛋白积累的增加上。另一方面，小鼠胚胎皮肤或肌肉成纤维细胞或椎骨成软骨细胞并没有响应 POMC 衍生肽而增加增殖。 ACTH 刺激成肌细胞增殖的半最大剂量约为 5 nM，并且次优剂量的成纤维细胞生长因子的促有丝分裂作用加倍。
Peptides derived from proopiomelanocortin (POMC) have been found to stimulate the proliferation of murine myogenic cells. Among these peptides, adrenocorticotropin (ACTH) and .alpha.-, .beta.-, and .gamma.-melanocyte-stimulating hormones (MSH) were found to be active, whereas the opioid peptides were not. At clonal density, both ACTH and MSH caused a three- to fourfold increase in the average number of cells per clone in myogenic but not in fibroblast colonies. At high cell density, ACTH and MSH caused a three- to fourfold increase in proliferation of myogenic cells, reflected by an increased accumulation of skeletal myosin. On the other hand hand mouse embyro skin or muscle fibroblasts or vertebral chondroblasts did not incease proliferation in response to POMC-derived peptides. The half-maximal dose at which ACTH stimulated myoblast proliferation was around 5 nM, and the mitogenic effect was doubled by suboptimal doses of fibroblast growth factor.