In vitro and in vivo effects of potassium and magnesium on storage up to 7 days of apheresis platelet concentrates in platelet additive solution
In vitro and in vivo effects of potassium and magnesium on storage up to 7 days of apheresis platelet concentrates in platelet additive solution
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DOI：
10.1111/j.1423-0410.2007.01002.x
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发表时间：
2008-02-01
期刊：
影响因子：
2.7
通讯作者：
Shanwell, A.
中科院分区：
文献类型：
--
作者：
Diedrich, B.;Sandgren, P.;Shanwell, A.
背景与目的将血小板储存时间延长至7天，可改善血小板的可用性、后勤管理并减少浪费。除了细菌检测方法外，在血小板储存液（SSP+）中添加镁和钾可以进一步提高延长储存血小板的质量。材料与方法将10名供者的单采血小板分离保存在两种不同的血小板添加溶液（Intersol和SSP+）中进行配对比较。在第1天和第7天后进行各种体外血小板功能和代谢测定。在体内研究中，血小板在储存7天后用（111）铟或（51）铬标记，并注射到相应的供体中。连续抽取血液样本进行恢复和生存测量。结果SSP+的体外参数显示糖酵解（葡萄糖消耗降低，乳酸生成减少）显著降低，低渗休克反应（HSR）和形状变化反应程度升高，通过RANTES（激活、正常、T细胞表达和分泌）、CD62p和CD63表达降低血小板活化程度。与SSP+相比，Intersol在第7天的血小板回收率更高，为65 +/- 11 vs. 53 +/- 13% (P = 0.023)，生存率为4.2 +/- 1.9 vs. 3.6 +/- 1.4天。结论添加了钾和镁的PAS储存的血小板体外特性显示出更高的质量，但这并不能通过恢复和存活的体内参数来验证。
Background and Objective Prolonged storage of platelets up to 7 days provides improved availability, logistical management and decreased wastage. Beside methods of bacterial detection, addition of magnesium and potassium to the platelet storage solution (SSP+) may further improve the quality of platelets with extended storage.Materials and Methods Apheresis platelets from 10 donors were divided and stored in two different platelet additive solutions (PAS) (Intersol and SSP+) for a paired comparison. A variety of in vitro platelet function and metabolic assays were performed both on day 1 and after 7 days of storage. For in vivo study, platelets were labelled with either (111)Indium or (51)Chromium after 7 days of storage and were injected into the corresponding donor. Serial blood samples were drawn for recovery and survival measurements.Results In vitro parameters for SSP+ showed significantly reduced glycolysis (lower glucose consumption and decreased production of lactate), a higher hypotonic shock response (HSR) and the extent of shape change reactivity and a lower degree of platelet activation by means of RANTES (regulated on activation, normal, T cell-expressed, and secreted), CD62p and CD63 expression. Platelet recovery on day 7 was higher for Intersol as compared to SSP+, 65 +/- 11 vs. 53 +/- 13% (P = 0.023), and survival showed no difference 4.2 +/- 1.9 vs. 3.6 +/- 1.4 days.Conclusion In vitro characteristics of platelets stored in PAS with addition of potassium and magnesium indicated higher quality, but this could not be verified by the in vivo parameters by means of recovery and survival.