A new automated multiple allergen simultaneous test-chemiluminescent assay (MAST-CLA) using an AP720S analyzer
A new automated multiple allergen simultaneous test-chemiluminescent assay (MAST-CLA) using an AP720S analyzer
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DOI：
10.1016/j.cca.2009.01.014
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发表时间：
2009-04-01
影响因子：
5
通讯作者：
Kim, Hyon Sur
中科院分区：
文献类型：
--
作者：
Lee, Sungsil;Lim, Hwan Sub;Kim, Hyon Sur
背景：在特应性疾病的诊断中，过敏原检测是至关重要的一步。多变应原同时检测-荧光检测（MAST-CLA）是一种用于体外筛选变应原特异性IgE抗体的简单且非侵入性的方法。方法：使用常规手动MAST-CLA试剂盒和新的自动MAST-CLA方法（用于Optigen检测的自动AP 720 S系统; Hitachi Chemical Diagnostics，Inc.，美国同时。结果：吸入剂组的结果比食物组给出了相对较高的类水平结果。吸入组20例患者中的8例（40%）和食物组18例患者中的9例（47.4%）显示出2个系统之间的100%一致性。18例患者（90%）的吸入面板和16例患者（84.2%）的食物面板显示超过91%的concordance.Conclusions：这些结果表明，MAST-共轭亚油酸测定使用新的，自动化的AP 720 S分析仪表现良好，显示出较高的一致率与传统的MAST-共轭亚油酸。与手动MAST-CL 4相比，自动化AP 720 S系统的检测时间更短，使用更小的血清体积（500 μ l），沿着还有其他便利性。(c)2009 Elsevier B. V.保留所有权利。
Background: In the diagnosis of atopic diseases, allergen detection is a crucial step. Multiple allergen simultaneous test-chemiluminescent assay (MAST-CLA) is a simple and noninvasive method for in vitro screening of allergen-specific IgE antibodies.Methods: The Korean Inhalant Panel test on 20 patients and Food Panel test on 19 patients were performed using the conventional manual MAST-CLA kit and the new automated MAST-CLA method (automated AP720S system for the Optigen Assay; Hitachi Chemical Diagnostics, Inc., USA) simultaneously. The results were evaluated for positive reactivity and concordance.Results: The results of inhalant panel gave a relatively higher class level result than the food panel. The 8 patients out of 20 (40%) of the inhalation panel, and 9 patients out of 18 (47.4%) of the food panel showed 100% concordance between the 2 systems. Eighteen patients (90%) of the Inhalation Panel and sixteen patients (84.2%) of the Food Panel showed more than 91% concordance.Conclusions: These results suggest that the MAST-CLA assay using the new, automated AP720S analyzer performs well, showing a high concordance rate with conventional MAST-CLA. Compared to manual MAST-CL4 the automated AP720S system has a shorter assay time and uses a smaller serum volume (500 mu l) along with other conveniences. (c) 2009 Elsevier B.V. All rights reserved.