Metabolomic and transcriptomic investigation of the mechanism involved in enantioselective toxicity of imazamox in Lemna minor
Metabolomic and transcriptomic investigation of the mechanism involved in enantioselective toxicity of imazamox in Lemna minor
复制标题
青萍中咪草啶对映选择性毒性机制的代谢组学和转录组学研究
DOI：
10.1016/j.jhazmat.2021.127818
复制
发表时间：
2021-12-04
影响因子：
13.6
通讯作者：
Tan，Huihua
中科院分区：
文献类型：
--
作者：
Li，Rui;Luo，Chenxi;Tan，Huihua
咪草胺(Imazamox，IM)是一种广泛应用于农业的手性农药。目前，从对映体水平探讨咪唑莫司对水生植物的毒性机理的研究较少。本研究系统地研究了IM对水生植物小浮萍的毒性和生理生化系统的对映选择性影响。小浮萍的代谢学和转录学被用来确定潜在的毒性机制。外消旋、R-和-IM的7d EC50分别为0.036、0.035和0.203 mg/L，显示对映体选择性毒性。此外，IM还可引起Lemna的脂质过氧化和抗氧化损伤，并抑制靶标酶的活性。代谢学和转录学数据表明，r-IM干扰了少数浮萍在光合作用、谷胱甘肽代谢途径、戊糖磷酸途径、玉米素生物合成以及卟啉和叶绿素代谢过程中的差异表达基因和代谢产物；S-IM影响苯丙氨酸代谢、苯丙烷生物合成、玉米素生物合成和次生代谢产物的生物合成。外消旋IM影响手术中的碳固定、谷胱甘肽代谢途径、玉米素生物合成和磷酸戊糖途径。研究结果为揭示IM对小浮萍的对映体选择性毒性机制提供了新的思路，并为开展环境风险评价奠定了基础。
Imazamox (IM) is a chiral pesticide that has been widely used in agriculture. Currently, few studies have investigated the toxicity mechanisms of imazamox to aquatic macrophyte from the enantiomer level. In this study, the enantioselective effects of IM on the toxicity and physiological and biochemical system of aquatic macrophyte Lemna minor were systematically investigated. Metabolomic and transcriptomic forLemna minorwere used to identify potential mechanisms of toxicity. 7 d EC50s for racemic-,R-, andS-IM were 0.036, 0.035, and 0.203 mg/L, respectively, showing enantioselective toxicity. In addition, IM causedLemna minorlipid peroxidation and antioxidant damage, and inhibited the activities of the target enzymes. Metabolomic and transcriptomic data indicated thatR-IM interferenced differentially expressed genes and metabolites ofLemna minorwhich were enriched in carbon fixation during photosynthesis, glutathione metabolic pathway, pentose phosphate pathway, zeatin biosynthesis, and porphyrin and chlorophyll metabolism.S-IM affected phenylalanine metabolism, phenylpropanoid biosynthesis, zeatin biosynthesis and secondary metabolite biosynthesis. Racemic-IM influenced carbon fixation during operation, glutathione metabolic pathway, zeatin biosynthesis and pentose phosphate pathway. The results provide new insights into the enantioselective toxicity mechanisms of IM toLemna minor, and lay the foundation for conducting environmental risk assessments.