THE PLATELET GLYCOPROTEIN IA-IIA-ASSOCIATED BR-ALLOANTIGEN SYSTEM IS EXPRESSED BY CULTURED ENDOTHELIAL-CELLS
THE PLATELET GLYCOPROTEIN IA-IIA-ASSOCIATED BR-ALLOANTIGEN SYSTEM IS EXPRESSED BY CULTURED ENDOTHELIAL-CELLS
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DOI：
10.1111/j.1365-2141.1990.tb07798.x
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发表时间：
1990-08-01
影响因子：
6.5
通讯作者：
VONDEMBORNE, AEGK
中科院分区：
文献类型：
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作者：
GILTAY, JC;BRINKMAN, HJM;VONDEMBORNE, AEGK
为了了解内皮细胞和血小板膜糖蛋白(GP)Ia-IIa(VLA-2)复合体之间的免疫学关系，我们研究了内皮细胞GPIa-IIa是否能够表达血小板GPIa-IIa相关BR-同种异体抗原系统。因此，我们在同一个体的血小板上(通过单抗特异性固定血小板抗原的方法)和培养的脐静脉内皮细胞上(通过免疫沉淀实验)检测了BR系统的两种相同形式(BRA和BRB)的抗血清。研究了来自血小板BR(a+b+)的内皮细胞和来自血小板BR(a-b+)个体的内皮细胞。我们的结果表明，内皮细胞GPIa-IIa在表达Bra和BRb同种异体抗原方面与血小板GPIa-IIa没有区别。BR同种异体抗原与内皮细胞GPIa-IIa的相关性被基于单抗特异性内皮细胞抗原固定(MAIEA)的检测结果所证实。这些数据进一步说明了血小板和内皮细胞GIPA-IIa(VLA-2)在结构和免疫学上的相似性。
To obtain information on the immunological relationship between the endothelial and platelet glycoprotein (GP)Ia-IIa (VLA-2) complex, we studied whether endothelial GPIa-IIa was able to express the platelet GPIa-IIa-associated Br-alloantigen system. Therefore, we tested antisera to both alleic forms of the Br system (Bra and Brb) on platelets (by an assay based on monoclonal antibody-specific immobilization of platelet antigens, MAIPA) and on cultured umbilical vein endothelial cells (by immunoprecipitation experiments) from the same individual. Endothelial cells from a platelet Br(a+b+), and from a platelet Br(a-b+) individual were studied. Our results indicate that endothelial GPIa-IIa is indistinguishable from platelet GPIa-IIa in its ability to express the Bra and Brb alloantigens. The association of Br alloantigens with endothelial GPIa-IIa was confirmed by the results of an assay based on monoclonal antibody-specific immobilization of endothelial antigens (MAIEA). These data further illustrate the structural and immunologic similarity of platelet and endothelial cell GIPa-IIa (VLA-2).