Identification of AtWRKY75 as a transcriptional regulator in the defense response to Pcc through the screening of Arabidopsis activation-tagged lines
Identification of AtWRKY75 as a transcriptional regulator in the defense response to Pcc through the screening of Arabidopsis activation-tagged lines
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DOI：
10.1007/s11816-013-0308-x
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发表时间：
2014-03-01
影响因子：
2.4
通讯作者：
Hwang, Duk-Ju
中科院分区：
文献类型：
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作者：
Choi, Changhyun;Park, Young Ho;Hwang, Duk-Ju
坏死营养型病原菌胡萝卜腐果胶杆菌（Pectobacterium carotovorum ssp.）胡萝卜腐菌（Pcc）在广泛的植物宿主中引起软腐病。用Pcc接种来自拟南芥激活标记系的约60，000个独立种子，并筛选抗性突变体。Rpe 1（抗性蛋白果胶杆菌1）突变体，其中有更多的抗Pcc比野生型（WT）的植物，选择进行进一步的研究。侧翼序列分析表明，Rpe 1的T-DNA插入位点位于V染色体中部。通过与T-DNA标签区域相邻的几个基因的表达分析，AtWRKY 75基因在Rpe 1突变体中的表达与WT植株相比高度上调。与WT植物相比，AtWRKY 75基因的上调显示与PDF1.2、VSP 1和PR 1基因的诱导相关。与WT相比，AtWRKY 75过表达系表现出减少的Pcc细菌生长。综上所述，我们的数据表明，AtWRKY 75应该是一个积极的监管机构在JA或SA介导的防御信号对PCC的反应。
The necrotrophic pathogen Pectobacterium carotovorum ssp. carotovorum (Pcc) causes soft rot in a broad range of plant hosts. Approximately 60,000 independent seeds from Arabidopsis activation tagging lines were inoculated with Pcc and screened for resistant mutants. An Rpe1 (resistance protein to Pectobacterium 1) mutant, which had more resistance to Pcc than wild-type (WT) plants, was selected for further study. The T-DNA inserting locus in Rpe1 was located on the middle of chromosome V by flanking sequence analysis. Through expression analysis with several genes adjacent to the T-DNA tagging region, AtWRKY75 gene was highly up-regulated in the Rpe1 mutant compared to the WT plant. The up-regulation of AtWRKY75 gene was shown to be correlated on the induction of the PDF1.2, VSP1 and PR1 genes compared to the WT plant. AtWRKY75 over-expression lines exhibited reduced Pcc bacterial growth compared to WT. Taken together, our data suggest that AtWRKY75 should be a positive regulator in the JA- or SA-mediated defense signaling responses to Pcc.