Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia
Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia
复制标题
DOI：
10.1038/s41598-017-08490-0
复制
发表时间：
2017-08-15
影响因子：
4.6
通讯作者：
Klitzman, Bruce
中科院分区：
文献类型：
--
作者：
Chien, Jennifer S.;Mohammed, Mahmoud;Klitzman, Bruce
新型可注射生物传感器被用于测量短暂性缺血之前、期间和之后的间质氧合。众所周知，缺血一段时间后会发生反应性充血。然而，血流增加并不一定意味着组织中氧分压增加。因此，本研究的目的是检验在释放后肢止血带闭塞后发生组织反应性高氧的假设。大鼠注射双侧后肢生物传感器，同时进行单侧股血管结扎。在大约一个月和三个月后，大鼠经历了一系列氧合挑战，包括短暂的后肢止血带闭塞。沿着生物传感器，近红外光谱法用于测量毛细血管中的氧合血红蛋白百分比，激光多普勒血流仪用于测量血流。观察到闭塞后反应性充血。伴有组织反应性高氧，证实闭塞后氧供应一定超过了预期的耗氧量增加。反应性高氧的生理现象的测量可以证明临床上有益的诊断和优化治疗。
Novel injectable biosensors were used to measure interstitial oxygenation before, during, and after transient ischemia. It is well known that reactive hyperemia occurs following a period of ischemia. However, increased blood flow does not necessarily mean increased oxygen tension in the tissue. Therefore, the purpose of this study was to test the hypothesis that tissue reactive hyperoxia occurs following release of hind-limb tourniquet occlusions. Rats were injected with bilateral hind-limb biosensors and were simultaneously subjected to a unilateral femoral vessel ligation. After approximately one and three months, the rats underwent a series of oxygenation challenges, including transient hind-limb tourniquet occlusion. Along with the biosensors, near infrared spectroscopy was used to measure percent oxyhemoglobin in capillaries and laser Doppler flowmetry was used to measure blood flow. Post-occlusion reactive hyperemia was observed. It was accompanied by tissue reactive hyperoxia, affirming that the post-occlusion oxygen supply must have exceeded the expected increased oxygen consumption. The measurement of the physiologic phenomenon of reactive hyperoxia could prove clinically beneficial for both diagnosis and optimizing therapy.