Adenosine kinase and 5′-nucleotidase activity after prolonged wakefulness in the cortex and the basal forebrain of rat
Adenosine kinase and 5′-nucleotidase activity after prolonged wakefulness in the cortex and the basal forebrain of rat
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DOI：
10.1016/s0197-0186(02)00155-9
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发表时间：
2003-05-01
影响因子：
4.2
通讯作者：
Porkka-Heiskanen, T
中科院分区：
文献类型：
--
作者：
Alanko, L;Heiskanen, S;Porkka-Heiskanen, T
本研究评估了长时间觉醒对腺苷激酶（AK）、外-5 '-核苷酸酶和内-5'-核苷酸酶活性的影响。将大鼠剥夺睡眠3或6 h，一组在剥夺6 h后允许睡眠2 h的恢复睡眠。解剖皮质和基底前脑，并在干冰上快速冷冻。腺苷激酶的酶活性通过监测[2-H-3]-腺苷转化为[H-3]-腺苷一磷酸（AMP）来测量，外-5 '-核苷酸酶和内-5'-核苷酸酶活性通过监测[2-H-3]-AMP转化为[H-3]-腺苷来测量。酶的活动没有改变剥夺或恢复睡眠时，无论是在皮层或基底前脑相比，未经处理的控制。在两个脑区的酶活性的显着昼夜变化。在基底前脑腺苷激酶和两种核苷酸酶显示其最低的活性在中间的休息阶段，6小时后的灯光，这表明一个低水平的腺苷代谢，在这个时间点的生产和降解。皮层腺苷激酶的昼夜活动模式与基底前脑相似，外-5 '-核苷酸酶的活性在静息期早期即开灯后3 h就已较低，并一直维持到静息期结束时，即开灯后8h，内-5'-核苷酸酶的活性无昼夜变化。这些活动模式可能与睡眠期间与清醒相比能量代谢水平较低有关。(C)2002爱思唯尔科技有限公司版权所有。
The effect of prolonged wakefulness on adenosine kinase (AK), ecto-5'-nucleotidase and endo-5'-nucleotidase activity was assessed in the present study. Rats were sleep deprived for 3 or 6 h, and one group was allowed to sleep 2 h of recovery sleep after the 6 h deprivation. The cortex and the basal forebrain were dissected, and frozen rapidly on dry ice. The enzyme activity of adenosine kinase was measured by monitoring the conversion of [2-H-3]-adenosine into [H-3]-adenosine monophosphate (AMP) and the ecto-5'-nucleotidase and endo-5'-nucleotidase activities by monitoring the conversion of [2-H-3]-AMP into [H-3]-adenosine. The enzyme activities did not change during deprivation or recovery sleep in either cortex or basal forebrain when compared to unhandled controls. Significant diurnal variation in enzyme activities was noted in both brain areas. In the basal forebrain adenosine kinase and both nucleotidases showed their lowest activity in the middle of the rest phase, 6 h after lights on, suggesting a low level of adenosine metabolism, both production and degradation at this time point. In the cortex adenosine kinase had a diurnal activity pattern similar to the basal forebrain and the ecto-5'-nucleotidase activity was low already early in the rest phase, 3 h after lights on, and remained low until the end part of the rest phase, 8 It after lights on. Endo-5'-nucleotidase lacked diurnal variation. These activity patterns may be associated with the lower level of energy metabolism during sleep compared to wakefulness. (C) 2002 Elsevier Science Ltd. All rights reserved.