A conserved LXXLF sequence is the major determinant in p6(gag) required for the incorporation of human immunodeficiency virus type 1 Vpr
A conserved LXXLF sequence is the major determinant in p6(gag) required for the incorporation of human immunodeficiency virus type 1 Vpr
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DOI：
10.1128/jvi.70.1.159-164.1996
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发表时间：
1996-01-01
影响因子：
5.4
通讯作者：
Gottlinger, HG
中科院分区：
文献类型：
--
作者：
Kondo, E;Gottlinger, HG
人类免疫缺陷病毒1型（HIV-1）的vpr基因产物是一种病毒相关调控蛋白。Vpr的可转移病毒粒子结合基序位于HIV-1的p6结构域。呕吐多蛋白。为了绘制p6中参与Vpr结合的序列，我们分析了p6突变形式指导Vpr结合到嵌合病毒颗粒中的能力。我们的研究结果表明，控制Vpr结合的决定因素主要局限于p6结构域的c端区域。在这个区域内，高度保守的序列基序（L-X-S-L-F-G）中的三个疏水残基是绝对需要的。值得注意的是，将保守基序和单个侧翼残基转移到异源Gag多蛋白上，足以在中等水平上转移整合Vpr的能力。p6残基32至46的转移导致Vpr的整合水平与全长HIV-1 Gag蛋白相当，表明该区域基本上包含有效整合Vpr所需的所有信息。
The vpr gene product of human immunodeficiency virus type 1 (HIV-1) is a virion-associated regulatory protein. A transferable virion association motif for Vpr is located in the p6 domain of the HIV-1. Gag polyprotein. To map the sequences in p6 that are involved in Vpr incorporation, we analyzed the ability of mutant forms of p6 to direct the incorporation of Vpr into chimeric viral particles. Our results show that the determinants which govern Vpr incorporation are largely confined to a C-terminal region of the p6 domain. Within this region, three hydrophobic residues in a highly conserved sequence motif (L-X-S-L-F-G) are absolutely required. Remarkably, the transfer of the conserved motif and of a single flanking residue to a heterologous Gag polyprotein was sufficient to transfer the ability to incorporate Vpr at moderate levels. The transfer of residues 32 to 46 of p6 led to Vpr incorporation levels that were comparable to those obtained with full-length HIV-1 Gag protein, indicating that this region contains essentially all the information required for efficient Vpr incorporation.