Insights into extracellular polymeric substances of cyanobacterium Microcystis aeruginosa using fractionation procedure and parallel factor analysis.
Insights into extracellular polymeric substances of cyanobacterium Microcystis aeruginosa using fractionation procedure and parallel factor analysis.
复制标题
DOI：
10.1016/j.watres.2013.01.019
复制
发表时间：
2013-04
期刊：
影响因子：
12.8
通讯作者：
Huacheng Xu;Haiyuan Cai;Guanghui Yu;Helong Jiang
中科院分区：
文献类型：
--
作者：
Huacheng Xu;Haiyuan Cai;Guanghui Yu;Helong Jiang
研究铜绿微囊藻的胞外聚合物（EPS）是了解铜绿微囊藻生长和增殖的关键。本研究采用分级分离和平行因子分析相结合的方法对M.铜绿。理化分析表明，无论生长阶段和培养基营养水平如何，胞外多糖基质中多糖的含量均高于蛋白质。在指数生长期，有机质主要分布在紧密结合的EPS（TB-EPS）组分中，而在衰减期，有机质急剧释放到可溶性EPS（SL-EPS）和松散结合的EPS（LB-EPS）组分中。应用荧光激发-发射矩阵（EEM）来表征EPS基质中的特定成分，收集的所有荧光EEM光谱均可通过PARAFAC分析成功分解为四组分模型。组分1 [激发/发射（Ex/Em）= 220/340]、组分2（Ex/Em = 280/340）和组分3 [Ex/Em =（200，220，270）/296]属于蛋白质类物质，而组分4 [Ex/Em =（250，340）/438]属于腐殖酸类物质。Pearson相关分析表明，LB-EPS和TB-EPS组分中的类胡萝卜素物质与微囊藻的生长呈正相关，而SL-EPS组分中的类胡萝卜素物质和类腐殖酸物质与微囊藻的生长呈正相关。铜绿。根据分离过程和EEM-PARAFAC分析结果，提出了微囊藻生长和增殖的科学意义。
Investigations on the extracellular polymeric substances (EPS) are crucial for better understanding the growth and proliferation of cyanobacterium Microcystis aeruginosa. In this study, a combined approach of fractionation procedure and parallel factor (PARAFAC) analysis were applied to characterize the EPS of M. aeruginosa. Physicochemical analysis showed that the contents of polysaccharides in EPS matrix were higher than those of proteins, regardless of the differences in growth phases and nutritional levels in medium. Organic matters were mainly distributed in the tightly bound EPS (TB-EPS) fraction during the exponential growth phase, whereas they sharply released to the soluble EPS (SL-EPS) and loosely bound EPS (LB-EPS) fractions at the decay period. Fluorescence excitation–emission matrix (EEM) was applied to characterize the specific compositions in EPS matrix, and all the fluorescence EEM spectra collected could be successfully decomposed into a four-component model by PARAFAC analysis. Component 1 [excitation/emission (Ex/Em) = 220/340], component 2 (Ex/Em = 280/340) and component 3 [Ex/Em = (200, 220, 270)/296] were attributed to protein-like substances, while component 4 [Ex/Em = (250, 340)/438] belonged to humic-like substances. Pearson correlation analysis demonstrated that tryptophan-like substances in the LB-EPS and TB-EPS fractions were positively correlated with Microcystis growth, whereas in the SL-EPS fraction, tryptophan-like as well as humic-like substances were associated with the growth of M. aeruginosa. The scientific implication for Microcystis growth and proliferation, based on the results of fractionation procedure and EEM-PARAFAC analysis, was also presented.