A new serum α-amylase assay of high sensitivity
A new serum α-amylase assay of high sensitivity
复制标题
一种新的高灵敏度血清 α-淀粉酶测定方法
DOI：
10.1016/0009-8981(77)90107-3
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
W. Whelan
中科院分区：
文献类型：
--
作者：
J. Marshall;A. P. Iodice;W. Whelan
本文介绍了用化学改性淀粉底物测定血清α-淀粉酶活性的方法。在过量的真菌葡糖淀粉酶存在下，α-淀粉酶对这些底物的作用导致葡萄糖的产生与存在的α-淀粉酶的量成正比。产生的葡萄糖通过特定的酶测定来测量。通过使用这种新的测定法获得的结果与通过常规的糖化测定法确定的活性良好相关。与大多数其他α-淀粉酶测定方法相比，新方法具有更高的灵敏度，并且不易受干扰。
The use of chemically modified starch substrates for measurement of serum α-amylase activity is described. Action of α-amylase on such substrates, in the presence of excess fungal glucoamylase, results in the production of glucose in direct proportion to the amount of α-amylase present. The glucose produced is measured by a specific enzymic assay. Results obtained by using this new assay correlate well with activities determined by a conventional saccharogenic assay. The new method is of much higher sensitivity, and is less susceptible to interference, than are most other α-amylase assay methods.