Molecular and phylogenetic analysis of MADS-box genes of MIKC type and chromosome location of SEP-like genes in wheat (Triticum aestivum L.)
Molecular and phylogenetic analysis of MADS-box genes of MIKC type and chromosome location of SEP-like genes in wheat (Triticum aestivum L.)
复制标题
DOI：
10.1007/s00438-007-0285-2
复制
发表时间：
2007-12-01
影响因子：
3.1
通讯作者：
Ciaffi, Mario
中科院分区：
文献类型：
--
作者：
Paollacci, Anna Rita;Tanzarella, Oronzo A.;Ciaffi, Mario
由mikc型MADS-box基因编码的转录因子控制着植物的许多重要功能，包括花的发育和形态发生。报道了普通小麦45个mikc型MADS-box全长cDNA序列的克隆与鉴定。利用已知的mikc型基因序列检索小麦EST数据库，设计引物进行RT-PCR克隆。通过5‘和3’ RACE延伸获得全长cdna。Southern分析表明，存在三个拷贝的MIKC序列，对应于三个同源基因。对6个sep样基因的非整倍体分析进一步证实了这种基因组组织，每个基因显示三个拷贝位于不同的同源染色体上。系统发育分析将小麦的MIKC cdna划分为植物中鉴定的13个MIKC亚类中的I - I个，与大多数控制花同源功能的基因相对应。采用RT-PCR、实时RT-PCR和北方杂交等方法，分析了18个小麦组织和花器官中不同同型同源分类对应的cdna的表达谱。根据序列同源性和与拟南芥和单子叶植物MADS-box基因的表达模式比较，预测了克隆小麦cdna对应基因的潜在功能。
Transcription factors encoded by MIKC-type MADS-box genes control many important functions in plants, including flower development and morphogenesis. The cloning and characterization of 45 MIKC-type MADS-box full-length cDNA sequences of common wheat is reported in the present paper. Wheat EST databases were searched by known sequences of MIKC-type genes and primers were designed for cDNA cloning by RT-PCR. Full-length cDNAs were obtained by 5' and 3' RACE extension. Southern analysis showed that three copies of the MIKC sequences, corresponding to the three homoeologous genes, were present. This genome organization was further confirmed by aneuploid analysis of six SEP-like genes, each showing three copies located in different homoeologous chromosomes. Phylogenetic analysis included the wheat MIKC cDNAs into I I of the 13 MIKC subclasses identified in plants and corresponding to most genes controlling the floral homeotic functions. The expression patterns of the cDNAs corresponding to different homeotic classes was analysed in 18 wheat tissues and floral organs by RT-PCR, real time RT-PCR and northern hybridisation. Potential functions of the genes corresponding to the cloned wheat cDNAs were predicted on the basis of sequence homology and comparable expression pattern with functionally characterized MADS-box genes from Arabidopsis and monocot species.