Plasma antioxidants and oxidative stress status in obese women: correlation with cardiopulmonary response
Plasma antioxidants and oxidative stress status in obese women: correlation with cardiopulmonary response
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DOI：
10.7717/peerj.9230
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发表时间：
2020-05-19
期刊：
影响因子：
2.7
通讯作者：
Aziz, Azlina Abdul
中科院分区：
文献类型：
--
作者：
Adenan, Dyg Mastura;Jaafar, Zulkarnain;Aziz, Azlina Abdul
导论.高体脂加上心肺功能低下和氧化应激增加被认为是心血管合并症的促成因素。本研究旨在探讨不同体重指数（BMI）的女性抗氧化剂和氧化应激状态与心肺反应的相关性。招募了80名女性成年人，并根据其BMI分为三组：正常体重（n = 23），超重（n = 28）和肥胖（n = 29）。在心肺运动试验前采集血样。通过离心分离血浆样品，并分析酶抗氧化活性，包括过氧化氢酶，谷胱甘肽过氧化物酶和超氧化物歧化酶。采用2，2 '-连氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除和血浆铁还原能力（FRAP）测定评估非酶抗氧化活性。为了评估受试者的氧化应激状态，测定了活性氧和脂质过氧化副产物丙二醛的水平。采用心肺运动试验（CPET）分析患者的心肺功能，包括峰值耗氧量、代谢当量和呼吸交换率等15个参数。肥胖组ABTS自由基清除率和FRAP活性明显低于正常体重组。肥胖组过氧化氢酶活性高于正常体重组。斯皮尔曼相关分析显示过氧化氢酶与峰值耗氧量呈负相关，偏相关分析显示超氧化物歧化酶与呼吸频率、ABTS活性与氧脉搏、心输出量呈负相关。我们的研究结果表明，肥胖女性的心血管健康和抗氧化能力较低;过氧化氢酶活性较高可能是一种补偿机制。这两个参数之间的负相关性可能表明抗氧化剂对心肺系统的潜在影响，值得在更大的人群中进一步分析。尽管如此，本研究为进一步探讨氧化还原状态与心肺反应之间的关系提供了基础。这可能用于预测未来发生与氧化应激相关疾病的风险，特别是肺和心血管疾病。
Introduction. A high body fat coupled with low cardiopulmonary fitness and an increase in oxidative stress has been connoted as contributing factors in developing cardiovascular comorbidities. This study aimed to investigate the correlation between antioxidants and oxidative stress status with cardiopulmonary responses in women of different body mass index (BMI).Subjects and Methods. Eighty female adults were recruited and divided into three groups; normal weight (n = 23), overweight (n = 28) and obese (n = 29), according to their BMI. Blood samples were obtained prior to cardiopulmonary exercise testing. Plasma samples were separated by centrifugation and analysed for enzymatic antioxidant activity including catalase, glutathione peroxidase and superoxide dismutase. Non-enzymatic antioxidant activities were assessed using 2, 2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging and ferric reducing ability of plasma (FRAP) assays. To evaluate the oxidative stress status of subjects, levels of reactive oxygen species and malondialdehyde, the by-product of lipid peroxidation, were measured. Cardiopulmonary responses were analysed using cardiopulmonary exercise testing (CPET) which involved 15 various parameters such as peak oxygen consumption, metabolic equivalents and respiratory exchange ratio.Results. The obese group had significantly lower ABTS radical scavenging and FRAP activities than the normal weight group. A higher catalase activity was observed in the obese group than the normal weight group. Spearman's correlation showed an inverse relationship between catalase and peak oxygen consumption, while partial correlation analysis showed inverse correlations between superoxide dismutase and respiratory frequency, ABTS activity and oxygen pulse, and between ABTS activity and cardiac output.Conclusion. Our results demonstrate a lower cardiovascular fitness and antioxidant capacity in obese women; the higher catalase activity may be a compensatory mechanism. The negative correlations found between these two parameters may indicate the potential effect of antioxidants on the cardiopulmonary system and deserve further analysis in a larger population. Nevertheless, this study provides the basis for future studies to further explore the relationships between redox status and cardiopulmonary responses. This can potentially be used to predict future risk of developing diseases associated with oxidative stress, especially pulmonary and cardiovascular diseases.