Introduction to High Performance Liquid Chromatography
Introduction to High Performance Liquid Chromatography
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DOI：
10.1007/978-94-009-5938-5_1
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
R. Hamilton;P. Sewell
中科院分区：
文献类型：
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作者：
R. Hamilton;P. Sewell
色谱法以其多种形式被广泛用作分离和分析技术。自James和Martin [1]引入气相色谱法以来，气相色谱法在该领域一直处于领先地位。纸色谱、薄层色谱、离子交换色谱和排阻色谱（凝胶渗透和凝胶过滤）形式的液相色谱不能取得同样的成功，主要是因为低的移动的流速导致的低效率和长的分析时间。液相色谱法的出现与气相色谱法相当，通常被认为始于Huber和Hulsman [2]在1967年的出版物，尽管Giddings [3]已经表明液相色谱法在柱效率和分析速度方面优于气相色谱法。
Chromatography in its many forms is widely used as a separative and an analytical technique. Gas chromatography since its introduction by James and Martin [1] has been pre-eminent in the field. Liquid chromatography in the form of paper, thin-layer, ion-exchange, and exclusion (gel permeation and gel filtration) chromatography had not been able to achieve the same success, mainly because of the poor efficiences and the long analysis times arising from the low mobile phase flow rates. The emergence of liquid chromatography on a basis comparable to gas chromatography is usually considered to start with the publication by Huber and Hulsman [2] in 1967, although Giddings [3] had already shown the potential advantage, in terms of column efficiencies and speed of analysis, of liquid chromatography over gas chromatography.