In vitro cell response and in vivo primary osteointegration of highly porous Ta-Nb alloys as implant materials
In vitro cell response and in vivo primary osteointegration of highly porous Ta-Nb alloys as implant materials
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DOI：
10.1002/jbm.b.34149
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发表时间：
2019-04-01
影响因子：
3.4
通讯作者：
Ruan, Jianming
中科院分区：
文献类型：
--
作者：
Liu, Jue;Zhou, Xiongwen;Ruan, Jianming
结合Ta的生物相容性和Nb的优异性能，成功制备了具有三维网络结构的α-型Ta-xNb（x=5，10和15wt%）合金。本工作旨在对多孔钽铌合金的体内外生物相容性进行系统研究。在本研究中，体外生物学反应的特点是细胞附着，细胞毒性试验，和基因表达。细胞在多孔合金表面分布均匀，块体/多孔Ta-15 Nb合金均表现出0-1的RGR水平。此外，上调的基因表达可能揭示骨再生的增加。通过组织学评价和拔出试验评估体内原发性成骨反应。体内结果表明，多孔Ta-15 Nb合金显示出比本体Ta-15 Nb合金更高的拔出力，并且在12周后显示出植入物与骨之间更广泛的接触。实验结果表明，多孔钽铌合金支架具有良好的生物相容性，具有良好的生物医学应用前景。(c)2018 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，2018。(c)2018 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater 107 B：573-581，2019。
By combining the biocompatibility of Ta and excellent performance of Nb, the -type Ta-xNb (x=5, 10, and 15 wt %) alloys with three-dimensional network structure was successfully fabricated. This work was designed to carry out the systematic study of in vitro/vivo biocompatibility of highly porous Ta-Nb alloys. In the present study, the in vitro biological response was characterized by cell attachment, cytotoxicity test, and gene expression. Cells were well distributed on porous alloy surface and both bulk/porous Ta-15Nb alloys showd a 0-1 RGR level. Moreover, the upregulated gene expression may reveal an increase in bone regeneration. An assessment of primary osteogenic response in vivo was achieved by histological evaluation and pull-out test. In vivo results demonstrated that the porous Ta-15Nb alloy showed a higher pull-out force than bulk Ta-15Nb alloy and exhibited a more extensive contact between implants and bones after 12 weeks. Based on the present experiments, porous Ta-Nb alloy scaffolds had a good biocompatibility and were promising for biomedical applications. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2018. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 573-581, 2019.