The BTB domains of the potassium channel tetramerization domain proteins prevalently assume pentameric states
The BTB domains of the potassium channel tetramerization domain proteins prevalently assume pentameric states
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DOI：
10.1002/1873-3468.12203
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发表时间：
2016-06-01
期刊：
影响因子：
3.5
通讯作者：
Ciccarelli, Luciano
中科院分区：
文献类型：
--
作者：
Smaldone, Giovanni;Pirone, Luciano;Ciccarelli, Luciano
含钾通道四聚化结构域（KCTD）蛋白参与基本的生理病理过程。在这里，我们报告的低聚状态的砖，电车轨道，广泛的复杂（BTB）域的七个不同的KCTD属于五个主要分支的家庭进化树的分析。尽管它们的功能和序列的变异性，目前的电子显微镜数据突出了定义明确的五聚体状态的所有域的发生。我们的数据还表明，这些国家共存的替代形式，其中包括开放的五聚体。使用KCTD 1作为模型进行的热变性分析表明，在这些蛋白质中，不同的结构域对其整体稳定性进行了合作。最后，KCTD 6 BTB与Cullin 3复合的负染电子显微照片显示存在具有五尖风车形状的组装体。
Potassium channel tetramerization domain-containing (KCTD) proteins are involved in fundamental physio-pathological processes. Here, we report an analysis of the oligomeric state of the Bric-a-brack, Tram-track, Broad complex (BTB) domains of seven distinct KCTDs belonging to five major clades of the family evolution tree. Despite their functional and sequence variability, present electron microscopy data highlight the occurrence of well-defined pentameric states for all domains. Our data also show that these states coexist with alternative forms which include open pentamers. Thermal denaturation analyses conducted using KCTD1 as a model suggest that, in these proteins, different domains cooperate to their overall stability. Finally, negative-stain electron micrographs of KCTD6 BTB in complex with Cullin3 show the presence of assemblies with a five-pointed pinwheel shape.