Antiviral and Immunoregulatory Role Against PCV2 in Vivo of Chinese Herbal Medicinal Ingredients.
Antiviral and Immunoregulatory Role Against PCV2 in Vivo of Chinese Herbal Medicinal Ingredients.
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中药成分体内抗PCV2病毒及免疫调节作用
DOI：
10.1515/jvetres-2017-0062
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发表时间：
2017-12
影响因子：
1.8
通讯作者：
Hu Y
中科院分区：
文献类型：
--
作者：
Yang H;Chen X;Jiang C;He K;Hu Y
摘要简介：研究中草药中常见的柴胡皂苷A、柴胡皂苷D、三七皂苷、三七皂苷R1和知母皂苷B4的抗病毒和免疫调节作用。材料和方法：用0.2mL含有107 TCID 50病毒/mL的猪圆环病毒2（PCV 2）溶液肌内（im）攻击对照小鼠。高、中和低剂量皂苷组的小鼠最初用0.2 mL PCV 2溶液im攻击，三天后用三种剂量（10、5或1 mg/kg b.w.）之一的五种皂苷之一腹膜内（ip）处理。在药物对照组中，小鼠以10 mg/kg b.w.空白对照组给予等体积生理盐水。结果如下：结果表明，皂苷可以降低PCV 2诱导的免疫病理损伤的发生率和严重程度，如体温升高、体重减轻、贫血和内脏肿胀。此外，观察到皂苷可影响免疫球蛋白水平和蛋白吸收。结论：人参皂甙具有免疫调节作用。
Abstract Introduction: The aim of the research was to investigate the antiviral and immunoregulatory effects of saikosaponin A, saikosaponin D, Panax notoginseng saponins, notoginsenoside R1, and anemoside B4 saponins commonly found in Chinese herbal medicines. Material and Methods: control mice were challenged intramuscularly (im) with 0.2 mL of porcine circovirus 2 (PCV2) solution containing 107 TCID50 of the virus/mL. Mice of high-, middle-, and low-dose saponin groups were initially challenged im with 0.2 mL of PCV2 solution and three days later treated intraperitoneally (ip) with one of five saponins at one of three doses (10, 5, or 1 mg/kg b.w.). In the drug control group, mice were dosed ip with 10 mg/kg b.w. of a given saponin, and mice in a blank control group were administered the same volume of normal saline. Results: The results revealed that the saponins could reduce the incidence and severity of PCV2-induced immunopathological damage, e.g. body temperature elevation, weight loss, anaemia, and internal organ swelling. In addition, it was seen that the saponins could affect the immunoglobulin levels and protein absorption. Conclusion: The data suggested that the saponins might effectively regulate immune responses.