Using multifocal plane microscopy to reveal novel trafficking processes in the recycling pathway
Using multifocal plane microscopy to reveal novel trafficking processes in the recycling pathway
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DOI：
10.1242/jcs.116327
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发表时间：
2013-03-01
影响因子：
4
通讯作者：
Ward, E. Sally
中科院分区：
文献类型：
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作者：
Gan, Zhuo;Ram, Sripad;Ward, E. Sally
细胞生物学中的一个主要突出问题是缺乏对管泡运输载体 (TC) 对 3D 细胞环境中细胞内运输途径的贡献的了解。这主要是由于使用显微镜技术来追踪这些高度活动的小隔室所面临的挑战。在本研究中，我们使用具有局部光激活的多焦点平面显微镜来克服这些限制。使用这种方法，我们对构成受体 FcRn 循环途径的各个成分进行了表征。具体来说，已经定义了 TC 与较大的、相对静态的分选内体相交的几种不同途径。这些途径包括一种新颖的“循环”过程，其中 TC 离开并返回到相同的分选内体。值得注意的是，具有不同行程的 TC 可以通过与 Rab GTPases、APPL1 和 SNX4 的不同互补体的关联来识别。这些研究为进一步分析回收途径提供了框架。
A major outstanding issue in cell biology is the lack of understanding of the contribution of tubulovesicular transport carriers (TCs) to intracellular trafficking pathways within 3D cellular environments. This is primarily due to the challenges associated with the use of microscopy techniques to track these highly motile, small compartments. In the present study we have used multifocal plane microscopy with localized photoactivation to overcome these limitations. Using this approach, we have characterized individual components constituting the recycling pathway of the receptor FcRn. Specifically, several different pathways followed by TCs that intersect with larger, relatively static sorting endosomes have been defined. These pathways include a novel 'looping' process in which TCs leave and return to the same sorting endosome. Significantly, TCs with different itineraries can be identified by associations with distinct complements of Rab GTPases, APPL1 and SNX4. These studies provide a framework for further analyses of the recycling pathway.