Monomeric IgE enhances human mast cell chemokine production: ILA-4 augments and dexamethasone suppresses the response
Monomeric IgE enhances human mast cell chemokine production: ILA-4 augments and dexamethasone suppresses the response
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DOI：
10.1016/j.jaci.2005.08.042
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发表时间：
2005-12-01
影响因子：
14.2
通讯作者：
Galli, SJ
中科院分区：
文献类型：
--
作者：
Matsuda, K;Piliponsky, AM;Galli, SJ
背景资料：小鼠IgE单克隆抗体能促进培养的小鼠骨髓源性肥大细胞的存活，并在缺乏已知特异性抗原的情况下诱导肥大细胞分泌介质目的：探讨人IgE在缺乏已知特异性抗原的情况下对肥大细胞分泌介质或存活的影响。我们测试了人IgE是否诱导人脐带血来源的肥大细胞分泌介质或增强其在干细胞因子撤回时的存活。暴露于浓度低至2.5 μ g/mL的IgE（而非IgG）可显著增强IL-8和单核细胞趋化蛋白1的释放，但不增强组胺或半胱氨酰白三烯的释放。然而，在测试的条件下，响应于IgE单独的趋化因子的产生显着低于诱导时，相同的IgE致敏的人肥大细胞的群体的等分试样与抗IgE刺激。IL-8和单核细胞趋化蛋白1的生产响应于单独的IgE或IgE和抗IgE的细胞在IL-4中预孵育增强，并通过用地塞米松预孵育细胞来抑制。相比之下，我们没有检测到任何能力的IgE，以提高肥大细胞的生存撤回的干细胞factor.Conclusion：暴露于人IgE在体外已知的特异性抗原的情况下，可以提高人肥大细胞的趋化因子的生产，这种分泌反应可以增强预孵育的肥大细胞与IL-4，可以抑制地塞米松。
Background: Mouse monoclonal IgE antibodies can promote the survival of mouse bone marrow-derived cultured mast cells and induce the cells to secrete mediators in the absence of known specific antigen.Objective: To determine whether human IgE, in the absence of known specific antigen, had effects on the mediator secretion or survival of human mast cells.Methods: We tested whether human IgE induced human cord blood-derived mast cells to secrete mediators or enhanced their survival on withdrawal of stem cell factor.Results: Exposure to IgE, but not IgG, at concentrations as low as 2.5 mu g/mL significantly enhanced the release of IL-8 and monocyte chemoattractant protein 1, but not histamine or cysteinyl leukotrienes. However, under the conditions tested, chemokine production in response to IgE alone was significantly less than that induced when aliquots of the same IgE-sensitized populations of human mast cells were stimulated with anti-IgE. The production of IL-8 and monocyte chemoattractant protein 1 in response to either IgE alone or IgE and anti-IgE was enhanced by preincubation of the cells in IL-4 and was inhibited by preincubation of the cells with dexamethasone. By contrast, we did not detect any ability of IgE to enhance mast cell survival on withdrawal of stem cell factor.Conclusion: Exposure to human IgE in vitro in the absence of known specific antigen can enhance chemokine production by human mast cells, and this secretory response can be enhanced by preincubation of the mast cells with IL-4 and can be suppressed by dexamethasone.