Laser induced autofluorescence diagnosis of bladder tumors: Dependence on the excitation wavelength
Laser induced autofluorescence diagnosis of bladder tumors: Dependence on the excitation wavelength
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DOI：
10.1016/s0022-5347(01)65455-7
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发表时间：
1996-11-01
影响因子：
6.6
通讯作者：
Avrillier, S
中科院分区：
文献类型：
--
作者：
Anidjar, M;Ettori, D;Avrillier, S
目的：我们评估了激光诱导自体荧光光谱区分膀胱肿瘤性病变与正常或非特异性炎症mucosa.Materials和方法的能力：三种不同的脉冲激光激发波长依次为：308 nm，（氯化氙准分子激光），337 nm。（氮激光器）和480nm。（香豆素染料激光器）。激发光通过专门设计的多纤维导管递送，该多纤维导管连接到通过具有盐水冲洗的标准膀胱镜的工作通道引入的1mm芯直径的二氧化硅单纤维。将捕获的荧光聚焦到光学多通道分析仪检测系统上。在获得知情同意后和即将进行膀胱肿瘤经尿道切除术前，对25例患者的器械性能进行了评价。结果：在337和480 nm处，激发波长与正常尿路上皮粘膜相比，膀胱肿瘤的总体荧光强度明显降低，而与肿瘤阶段和级别无关。在308 nm处。激发波长包括原位癌在内的肿瘤光谱的形状明显不同于正常或非特异性炎症粘膜的形状。在这种情况下，没有绝对的强度测定，因为可以建立一个明确的诊断的基础上，在360和440 nm的荧光强度比。结论：这种光谱研究可能是特别有用的设计一个简化的自体荧光成像装置检测隐匿性尿路上皮肿瘤。
Purpose: We assessed the ability of laser induced autofluorescence spectroscopy to distinguish neoplastic urothelial bladder lesions from normal or nonspecific inflammatory mucosa.Materials and Methods: Three different pulsed laser excitation wavelengths were used successively: 308 nm, (xenium chloride excimer laser), 337 nm. (nitrogen laser) and 480 nm. (coumarin dye laser). The excitation light was delivered by a specially devised multifiber catheter connected to a 1 mm, core diameter silica monofiber introduced through the working channel of a standard cystoscope with saline irrigation. The captured fluorescence light was focused onto an optical multichannel analyzer detection system. Device performance was evaluated in 25 patients after obtaining consent and immediately before transurethral resection of a bladder tumor. Spectroscopic results were compared with histological findings.Results: At 337 and 480 nm. excitation wavelengths the overall fluorescence intensity of bladder tumors was clearly decreased compared to normal urothelial mucosa regardless of tumor stage and grade. At the 308 nm. excitation wavelength the shape of the tumor spectra, including carcinoma in situ, was markedly different from that of normal or nonspecific inflammatory mucosa. No absolute intensity determinations were required in this situation, since a definite diagnosis could be established based on the fluorescence intensity ratio at 360 and 440 nm.Conclusions: This spectroscopic study could be particularly useful design a simplified autofluorescence imaging device for detection of occult urothelial neoplasms.