Occult Hepatitis C Virus Infection in Iranian Patients With Cryptogenic Liver Disease
Occult Hepatitis C Virus Infection in Iranian Patients With Cryptogenic Liver Disease
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DOI：
10.1002/jmv.22044
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发表时间：
2011-06-01
影响因子：
12.7
通讯作者：
Alizadeh, Amir Houshang Mohammad
中科院分区：
文献类型：
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作者：
Bokharaei-Salim, Farah;Keyvani, Hossein;Alizadeh, Amir Houshang Mohammad
隐源性肝病的诊断是在经过广泛的评估后，排除了慢性肝病的可识别病因。在这项研究中，丙型肝炎病毒（HCV）RNA的存在进行了测试，从伊朗患者的外周血单核细胞（PBMC），虽然发现血浆HCV RNA和抗HCV抗体阴性，患有慢性肝病的病因不明。从2007年9月至2010年3月，来自德黑兰的69例病因不明的慢性肝病患者被转诊至我们中心，并入选本研究。从10 mL外周血样本中分离PBMC。通过逆转录聚合酶链反应（RT-PCR）检测血浆和PBMC样本中的HCV-RNA状态。用RT-PCR方法检测HCV阳性PBMC中HCV-RNA。采用限制性片段长度多态性（RFLP）分析HCV阳性标本中的HCV基因型，将PCR产物克隆入pJET 1.2/blunt克隆载体后，通过5'端非编码区测序确定HCV基因型。在69例患者中，7例（10%）PBMC标本中检测到HCV-RNA。HCV-RNA基因分型结果显示，隐匿性HCV感染者中1b型3例（43%），la型2例（29%），3a型2例（29%）。这项研究的结果表明，不明原因的慢性肝病患者可能有隐匿性HCV感染的抗-HCV抗体和血浆HCV-RNA的情况下。有人建议，在没有肝活检标本的情况下，PBMC样本的HCV-RNA分析将提供信息。医学病毒学杂志83：989-995，2011。(C)2011 Wiley-Liss，Inc.
The diagnosis of cryptogenic liver disease is made when after extensive evaluations, recognizable etiologies of chronic liver disease are excluded. In this study, the presence of hepatitis C virus (HCV) RNA was tested in peripheral blood mononuclear cells (PBMCs) taken from Iranian patients who although were found negative for plasma HCV RNA and anti-HCV antibodies, suffered from chronic liver disease of unknown etiology. From September 2007 to March 2010, 69 patients from Tehran with chronic liver disease of unknown etiology who were referred to our center were enrolled in the present study. PBMCs were isolated from 10 mL peripheral blood specimens. HCV-RNA status was tested in plasma and PBMCs samples by reverse-transcription polymerase chain reaction (RT-PCR). HCV-RNA was detected in HCV-positive PBMCs specimens by RT-PCR method. HCV genotypes were subsequently analyzed in HCV-positive samples using restriction fragment length polymorphism (RFLP) assay; then HCV genotypes were confirmed by sequencing of 5' non-coding fragments after cloning PCR products into pJET1.2/blunt cloning vector. HCV-RNA was detected in PBMCs specimens belonging to 7 (10%) out of 69 patients. Genotyping of the HCV-RNA isolated from PBMCs showed that 3 (43%) patients with occult HCV infection had genotype 1b, 2 (29%) had genotype la, and another 2 (29%) had genotype 3a. The results of this study suggest that patients with chronic liver disease of unknown etiology may have occult HCV infection in the absence of anti-HCV antibodies and plasma HCV-RNA. It has been suggested that in the absence of liver biopsy specimens, analysis of PBMC sample for HCV-RNA would be informative. J. Med. Virol. 83:989-995, 2011. (C) 2011 Wiley-Liss, Inc.