Impaired microvascular response to graded coronary occlusion in diabetic and hyperglycemic dogs.
Impaired microvascular response to graded coronary occlusion in diabetic and hyperglycemic dogs.
复制标题
糖尿病和高血糖狗对分级冠状动脉闭塞的微血管反应受损。
DOI：
10.1152/ajpheart.1995.268.4.h1667
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Dellsperger,KC
中科院分区：
文献类型：
--
作者：
Kersten,JR;Brooks,LA;Dellsperger,KC
在9例四氧嘧啶治疗（60 mg/kg i. v.）的糖尿病患者中测试了糖尿病患者对缺血的冠状动脉微血管反应受损的假设，8只高血糖犬和16只对照犬。采用频闪落射照明和荧光显微血管造影活体显微镜测量完整搏动左心室小动脉直径。在平均冠状动脉灌注压分级降低至60 +/- 1（SE）mmHg（轻度狭窄）、39 +/- 1 mmHg（重度狭窄）和26 +/- 1 mmHg（冠状动脉闭塞）期间测量冠状动脉直径。对照组、糖尿病组和高血糖组动物的血糖水平分别为95 +/- 5、264 +/- 17和277 +/- 15 mg/dl。在对照组犬中，小动脉微血管（< 100微米）扩张（轻度狭窄、重度狭窄和冠状动脉闭塞期间直径较基线分别变化24 +/- 5、31 +/- 5和26 +/- 6%）。糖尿病或高血糖阻止了正常的扩张反应，并导致灌注压降低期间微血管直径减小（糖尿病动物直径变化为-2 +/-3、-4 +/-3和-15 +/-4%，高血糖动物直径变化为-11 +/-2、-9 +/-4和-8 +/-5%）。糖尿病和高血糖动物的大血管（> 100微米）扩张也显着受损。在糖尿病和高血糖犬严重狭窄期间，心外膜心肌灌注显著低于对照犬。由于ATP敏感性K+（KATP）通道介导正常动物的这种反应，我们测试的假设，KATP通道的反应性受损，糖尿病和高血糖症。（250字处删节）
The hypothesis that coronary microvascular responses to ischemia are impaired in diabetes was tested in 9 alloxan-treated (60 mg/kg i.v.), 8 hyperglycemic, and 16 control dogs. Arteriolar diameters were measured in intact beating left ventricle by use of stroboscopic epi-illumination and intravital microscopy with fluorescence microangiography. Coronary arterial diameters were measured during graded reductions in mean coronary perfusion pressure to 60 +/- 1 (SE) mmHg (mild stenosis), 39 +/- 1 mmHg (severe stenosis), and 26 +/- 1 mmHg (coronary artery occlusion). Blood glucose levels were 95 +/- 5, 264 +/- 17, and 277 +/- 15 mg/dl in control, diabetic, and hyperglycemic animals, respectively. In control dogs, arteriolar microvessels (< 100 microns) dilated (24 +/- 5, 31 +/- 5, and 26 +/- 6% change in diameter from baseline during mild stenosis, severe stenosis, and coronary occlusion, respectively). Diabetes or hyperglycemia prevented the normal dilatory response and resulted in decreases in microvascular diameter during decreases in perfusion pressure (-2 +/- 3, -4 +/- 3, and -15 +/- 4% change in diameter in diabetic animals and -11 +/- 2, -9 +/- 4, and -8 +/- 5% change in diameter in hyperglycemic animals). Large-vessel (> 100 microns) dilation was also significantly impaired in diabetic and hyperglycemic animals. Myocardial perfusion was significantly lower in the epicardium during a severe stenosis in diabetic and hyperglycemic than in control dogs. Because the ATP-sensitive K+ (KATP) channel mediates this response in normal animals, we tested the hypothesis that KATP channel responsiveness is impaired in diabetes and hyperglycemia.(ABSTRACT TRUNCATED AT 250 WORDS)