A new individualization marker of sweat: deoxyribonuclease I (DNase I) polymorphism.
A new individualization marker of sweat: deoxyribonuclease I (DNase I) polymorphism.
复制标题
一种新的汗液个体化标记:脱氧核糖核酸酶 I (DNase I) 多态性。
DOI:
10.1520/jfs14012j
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发表时间:
1996
影响因子:
1.6
通讯作者:
K. Kishi
中科院分区:
文献类型:
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作者:
T. Yasuda;H. Takeshita;K. Sawazaki;D. Nadano;R. Iida;S. Miyahara;K. Kishi
We confirmed for the first time, both biochemically and immunologically, the existence of deoxyribonuclease I (DNase I) in human liquid sweat. Isoelectric focusing of sweat samples on polyacrylamide gels (pH 3.5 to 5), followed by dried agarose film overlay detection, was used to determine the phenotypes of sweat DNase I. Because this detection method not only had high sensitivity, but also high band resolution, it was possible to determine DNase I types from sweat samples of 50 to 100 microL. Pretreatment of sweat samples with sialidase was essential for typing to enhance markedly the sensitivity accompanied by simplification of the isozyme pattern. The DNase I types in all sweat samples were consistently related to the types found in corresponding blood, urine, and semen samples. DNase I typing could, therefore, provide a novel discriminant characteristic in the forensic examination of sweat.
DOI:
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发表时间:
2004
期刊:
日本臨床 62・増11
影响因子:
--
作者:
岸 紘一郎
通讯作者:
岸 紘一郎
DOI:
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发表时间:
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期刊:
影响因子:
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作者:
通讯作者:
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