Overexpression of uracil permease and nucleoside transporter from Bacillus amyloliquefaciens improves cytidine production in Escherichia coli
Overexpression of uracil permease and nucleoside transporter from Bacillus amyloliquefaciens improves cytidine production in Escherichia coli
复制标题
解淀粉芽孢杆菌尿嘧啶通透酶和核苷转运蛋白的过度表达可提高大肠杆菌中的胞苷产量
DOI：
10.1007/s10529-021-03103-3
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发表时间：
2021-03-01
影响因子：
2.7
通讯作者：
Zhang，Heng
中科院分区：
文献类型：
--
作者：
Ma，Ruoshuang;Fang，Haitian;Zhang，Heng
胞苷是核酸保健食品和基因工程研究的重要原料。近年来，它在抗病毒、抗肿瘤、抗艾滋病药物中显示出不可替代的作用。其生物合成途径复杂且受高度调控。本研究发现，在大肠杆菌BG-08中过量表达解淀粉芽孢杆菌的尿嘧啶渗透酶和核苷转运蛋白，可使摇瓶培养的胞苷产量分别比原菌株（0.70±0.03 g/L）提高1.3倍（0.91±0.03 g/L）和1.8倍（1.26±0.03 g/L）。尿嘧啶渗透酶和核苷转运蛋白的共过表达使胞苷产量比原菌株提高了2.7倍（1.59±0.05 g/L）。以上结果表明，尿嘧啶渗透酶和核苷转运蛋白的过表达可促进胞苷的积累，并且这两种蛋白在大肠杆菌胞苷的分泌中发挥协同作用。
Cytidine is an important raw material for nucleic acid health food and genetic engineering research. In recent years, it has shown irreplaceable effects in anti-virus, anti-tumor, and AIDS drugs. Its biosynthetic pathway is complex and highly regulated. In this study, overexpression of uracil permease and a nucleoside transporter fromBacillus amyloliquefaciensrelated to cell membrane transport inEscherichia colistrain BG-08 was found to increase cytidine production in shake flask cultivation by 1.3-fold (0.91 ± 0.03 g/L) and 1.8-fold (1.26 ± 0.03 g/L) relative to that of the original strain (0.70 ± 0.03 g/L), respectively. Co-overexpression of uracil permease and a nucleoside transporter further increased cytidine yield by 2.7-fold (1.59 ± 0.05 g/L) compared with that of the original strain. These results indicate that the overexpressed uracil permease and nucleoside transporter can promote the accumulation of cytidine, and the two proteins play a synergistic role in the secretion of cytidine inEscherichia coli.