A putative Drosophila pheromone receptor expressed in male-specific taste neurons is required for efficient courtship
A putative Drosophila pheromone receptor expressed in male-specific taste neurons is required for efficient courtship
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DOI：
10.1016/s0896-6273(03)00542-7
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发表时间：
2003-09-11
期刊：
影响因子：
16.2
通讯作者：
Amrein, H
中科院分区：
文献类型：
--
作者：
Bray, S;Amrein, H
在高等动物中繁殖需要高效和准确地展示先天交配行为。在果蝇中，雄性求爱由一系列刻板的行为组成，涉及多种感觉方式，如视觉、听觉和化学感觉。例如，位于男性前腿和嘴唇触须上的味觉刷毛被认为识别女性分泌的非挥发性信息素。在这里，我们报告了可能的信息素受体Gr68a的鉴定，它表达在大约20个男性特异性前腿味觉刷毛的化学感觉神经元中。Gr68a的表达依赖于性别决定基因Doublesex，它控制着性别分化的许多方面，是正常求偶行为所必需的。破伤风毒素介导的Gr68a表达神经元的失活或转基因RNA干扰Gr68a RNA导致雄性求偶行为显著降低，提示Gr68a蛋白是果蝇信息素驱动求偶行为的重要组成部分。
Propagation in higher animals requires the efficient and accurate display of innate mating behaviors. In Drosophila melanogaster, male courtship consists of a stereotypic sequence of behaviors involving multiple sensory modalities, such as vision, audition, and chemosensation. For example, taste bristles located in the male forelegs and the labial palps are thought to recognize nonvolatile pheromones secreted by the female. Here, we report the identification of the putative pheromone receptor GR68a, which is expressed in chemosensory neurons of about 20 male-specific gustatory bristles in the forelegs. Gr68a expression is dependent on the sex determination gene doublesex, which controls many aspects of sexual differentiation and is necessary for normal courtship behavior. Tetanus toxin-mediated inactivation of Gr68a-expressing neurons or transgene-mediated RNA interference of Gr68a RNA leads to a significant reduction in male courtship performance, suggesting that GR68a protein is an essential component of pheromone-driven courtship behavior in Drosophila.