Genetic subtyping and V3 serotyping of HIV type 1 isolates in Congo
Genetic subtyping and V3 serotyping of HIV type 1 isolates in Congo
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DOI：
10.1089/088922299311501
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发表时间：
1999-02-10
影响因子：
1.5
通讯作者：
Agut, H
中科院分区：
文献类型：
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作者：
Candotti, D;Tareau, C;Agut, H
1 型人类免疫缺陷病毒 (HIV-1) 显示出高度的遗传变异性，并且正在快速进化。系统发育分析将 HIV-1 分离株分类为具有相应亚型 A±J 的主要组 (M) 和异常组 (O)。 1 此外，据报道，一些分离株的系统发育分类“不确定”，或者是 M 组不同亚型之间重组的结果。 1, 2 迄今为止，人们对这些遗传亚型的生物学意义知之甚少。一般来说，遗传亚型是使用分子生物学方法确定的，例如gag和env测序或异源双链体迁移率测定。 3 此外，还使用 V3 血清分型进行了有关 HIV-1 亚型的大型流行病学研究。这些血清分型测定基于抗体与源自 env 遗传亚型的 V3 肽的结合来提供 HIV-1 分类。 4, 5 然而，尽管 HIV-1 血清分型已被证明适合研究 V3 血清型的频率分布，但其预测遗传亚型的能力受到质疑。 6HIV-1 亚型可能具有主要的地理分布，尽管也有遗传亚型地理混合的报道。 7 除 I 亚型外，所有 HIV-1 亚型均在 HIV-1 流行的撒哈拉以南非洲地区被发现。 8 先前的流行病学研究表明，西非（科特迪瓦、贝宁、冈比亚、加纳、几内亚比绍、尼日利亚、多哥）的主要 HIV-1 亚型为 A 亚型，东非（乌干达、卢旺达、肯尼亚、坦桑尼亚、布隆迪）的主要 HIV-1 亚型为 A 亚型，南部非洲（马拉维、赞比亚、津巴布韦、南非）和北非（埃塞俄比亚、吉布提）。 8 在中部非洲（喀麦隆、加蓬、中非共和国和前扎伊尔）观察到更复杂的亚型分布。 8 在该区域，A 亚型占主导地位，但也检测到所有其他亚型甚至 O 组分离株。据我们所知，刚果没有 HIV-1 亚型的数据，该地区接近 HIV-1 主要多样性地区。
THE HUMAN IMMUNODEFICIENCY VIRUS type 1 (HIV-1) displays a high genetic variability that is rapidly evolving. Phylogenetic analyses have resulted in the classification of HIV-1 isolates into a major group (M) with corresponding subtypes A±J, and an outlier group (O). 1 In addition, some isolates have been reported as ª uncertainº with respect to their phylogenetic classification or as the result of recombination between different subtypes of group M. 1, 2 So far, little is known about the biological significance of these genetic subtypes. Generally, genetic subtypes are determined using molecular biological methods such as gag and env sequencing or the heteroduplex mobility assay. 3 In addition, large epidemiological studies regarding HIV-1 subtypes have been performed using V3 serotyping. These serotyping assays provide an HIV-1 classification based on antibody binding to V3 peptides derived from env genetic subtypes. 4, 5 However, although HIV-1 serotyping has proved to be appropriate for studying the frequency distribution of V3 serotypes, its ability to predict genetic subtypes has been questioned. 6HIV-1 subtypes may have a predominant geographical distribution although geographic intermixing of genetic subtypes has been reported, too. 7 All the HIV-1 subtypes, except subtype I, have been detected in sub-Saharan Africa, where HIV-1 is endemic. 8 Previous epidemiologic studies have shown that the dominant HIV-1 subtypes were subtypes A in West Africa (Ivory Coast, Benin, Gambia, Ghana, Guinea-Bisseau, Nigeria, Togo), A and D in East Africa (Uganda, Rwanda, Kenya, Tanzania, Burundi), and C in southern Africa (Malawi, Zambia, Zimbabwe, South Africa) and northern Africa (Ethiopia, Djibouti). 8 A more complex subtype distribution was observed in Central Africa (Cameroon, Gabon, Central African Republic, and former Zaire). 8 In this area, subtype A predominated but all the other subtypes and even group O isolates were also detected. To our knowledge, there are no data on HIV-1 subtypes in Congo, which is close to this area of major HIV-1 diversity.