Relative permeability of nasal, tracheal, and bronchoalveolar mucosa to macromolecules in rats exposed to ozone.
Relative permeability of nasal, tracheal, and bronchoalveolar mucosa to macromolecules in rats exposed to ozone.
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暴露于臭氧的大鼠鼻腔、气管和支气管肺泡粘膜对大分子的相对渗透性。
DOI：
10.1080/15287398609530822
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Crocker,TT
中科院分区：
文献类型：
--
作者：
Bhalla,DK;Mannix,RC;Kleinman,MT;Crocker,TT
研究了臭氧急性暴露对大鼠鼻腔、气管和支气管肺泡损伤的通透性变化。将99mTc标记的二乙基三胺苯乙酸酯（DPTA）和125l标记的牛血清白蛋白（BSA）选择性地灌注到暴露于0.8 ppm o3或清洁空气中2小时的麻醉大鼠的局部气道区域。通过计数在注射后短时间间隔内收集的血液样本中的放射性来检测放射性标记示踪剂的粘膜转移。在o3暴露后的第0、1和2天进行渗透性测量，以分析鼻腔、气管和支气管肺泡区组织损伤的程度和持久性。正常鼻腔粘膜通透性低，支气管肺泡区中等，气管粘膜通透性高。通过通透性升高反映的O3相关损伤主要发生在气管和支气管肺泡区，但在暴露后立即发生在鼻子的损伤很小。气管通透性异常持续时间不超过24小时，支气管肺泡区通透性异常持续时间超过24小时。结果与o3对小气道和肺泡区的损伤大于对气管的损伤一致。
Nasal, tracheal and bronchoalveolar injuries resulting from acute ozone exposure of rats were investigated by permeability changes.99mTc‐labeled diethylenetriaminepen‐taacetate (DPTA) and125l‐labeled bovine serum albumin (BSA) were selectively instilled into localized airway regions of anesthetized rats exposed to 0.8 ppm O3or clean air for 2 h. Transmucosal transfer of the radiolabeled tracers was detected by counting the radioactivity in blood samples collected at short postinstillation time intervals. Permeability measurements were made on d 0, 1, and 2 after O3exposure to analyze the extent and persistence of tissue injury in the nasal, tracheal, and bronchoalveolar regions. Normal mucosal permeability was low in nose, intermediate in bronchoalveolar zone, and high in trachea. The O3‐related injury, reflected by elevated permeability, was substantial in the trachea and bronchoalveolar zone but was minimal in the nose immediately after the exposure. Abnormal permeability persisted for less than 24 h in the trachea but for more than 24 h in the bronchoalveolar zone. The results are consistent with the properties of O3of causing greater injury in the smaller airways and the alveolar zone than in the trachea.