Involvement of CmWRKY10 in Drought Tolerance of Chrysanthemum through the ABA-Signaling Pathway.
Involvement of CmWRKY10 in Drought Tolerance of Chrysanthemum through the ABA-Signaling Pathway.
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CmWRKY10 通过 ABA 信号通路参与菊花的耐旱性
DOI：
10.3390/ijms17050693
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发表时间：
2016-05-11
影响因子：
5.6
通讯作者：
Chen F
中科院分区：
文献类型：
--
作者：
Jaffar MA;Song A;Faheem M;Chen S;Jiang J;Liu C;Fan Q;Chen F
干旱是影响植物生长和产量的重要非生物因素之一。WRKY转录因子在植物的生长发育以及许多非生物胁迫的提高中起着关键作用。在WRKY家族的三个主要类群中，IIe WRKY组在花卉作物方面的研究最少。在这里，我们报道了IIe WRKY组成员的功能，即菊花中参与抗旱的CmWRKY10。反式激活实验表明，CmWRKY10在酵母细胞中具有转录活性，亚细胞定位表明其定位于细胞核。我们前期的研究表明，干旱可以诱导菊花中CmWRKY10的表达。此外，与野生型(WT)相比，CmWRKY10在转基因菊花植株中的过表达提高了对干旱胁迫的耐受性。DREB1A、DREB2A、CuZnSOD、NCED3A和NCED3B转录本在高表达植株中的高表达为抗旱机制与脱落酸(ABA)途径相关提供了强有力的证据。此外，CmWRKY10高表达品系的ROS积累量低于WT品系，而过氧化物酶、超氧化物歧化酶和过氧化氢酶活性高于WT品系，说明其在抗旱性中的作用。综上所述，这些发现揭示了CmWRKY10通过调节胁迫相关基因在干旱胁迫中发挥积极的调节作用。
Drought is one of the important abiotic factors that adversely affects plant growth and production. The WRKY transcription factor plays a pivotal role in plant growth and development, as well as in the elevation of many abiotic stresses. Among three major groups of the WRKY family, the group IIe WRKY has been the least studied in floral crops. Here, we report functional aspects of group IIe WRKY member, i.e., CmWRKY10 in chrysanthemum involved in drought tolerance. The transactivation assay showed that CmWRKY10 had transcriptional activity in yeast cells and subcellular localization demonstrated that it was localized in nucleus. Our previous study showed that CmWRKY10 could be induced by drought in chrysanthemum. Moreover, the overexpression of CmWRKY10 in transgenic chrysanthemum plants improved tolerance to drought stress compared to wild-type (WT). High expression of DREB1A, DREB2A, CuZnSOD, NCED3A, and NCED3B transcripts in overexpressed plants provided strong evidence that drought tolerance mechanism was associated with abscisic acid (ABA) pathway. In addition, lower accumulation of reactive oxygen species (ROS) and higher enzymatic activity of peroxidase, superoxide dismutase and catalase in CmWRKY10 overexpressed lines than that of WT demonstrates its role in drought tolerance. Together, these findings reveal that CmWRKY10 works as a positive regulator in drought stress by regulating stress-related genes.