The IgE/IgG binding capacity and structural changes of Alaska Pollock parvalbumin glycated with different reducing sugars
The IgE/IgG binding capacity and structural changes of Alaska Pollock parvalbumin glycated with different reducing sugars
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不同还原糖糖化阿拉斯加狭鳕小白蛋白的IgE/IgG结合能力及结构变化
DOI：
10.1111/jfbc.13539
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发表时间：
2020-10-26
影响因子：
4
通讯作者：
Li, Jin-lin
中科院分区：
文献类型：
--
作者：
Zhang, Min;Tu, Zong-cai;Li, Jin-lin
小白蛋白（PV）是鱼类的主要过敏原之一。本研究旨在研究不同还原糖（葡萄糖、果糖、核糖、乳糖和半乳糖）糖基化对阿拉斯加鳕鱼PV免疫球蛋白E （IgE）和免疫球蛋白G （IgG）结合能力和结构变化的影响机制。与核糖、半乳糖或乳糖相比，与葡萄糖或果糖糖化的PV- glu / PV- fru具有更高的IgE/IgG结合能力。在糖基化过程中，PV的赖氨酸（Lyr）、酪氨酸（Tyr）和苯丙氨酸（Phe）逐渐嵌入蛋白质三维结构的核心区域，这在紫外（UV）光谱和荧光光谱中有所体现。此外，表面疏水性的增加证实了糖基化PV的构象改变。这些结果表明，在糖基化过程中PV的变化与潜在的致敏性之间存在特定的关联。还原糖的种类和构象对PV的IgE/IgG结合能力有很大影响，用核糖和半乳糖糖化是降低阿拉斯加鳕鱼PV的IgE/IgG结合能力的一种很有前途的方法。小鱼白蛋白（PV）是鱼类的主要过敏原，它不仅能维持细胞的生理活性，还能与人类淀粉样蛋白发生交叉反应，缓解阿尔茨海默病和帕金森综合征。本研究揭示了阿拉斯加鳕鱼经不同还原糖基化修饰后的PV的IgE/IgG结合能力和结构变化。这将有助于我们了解PV与不同还原糖反应后糖基化产物的敏化和结构变化。为糖基化制备低抗原性PV提供了有效的羰基来源，为糖基化修饰其他食物过敏原奠定了基础。
Parvalbumin (PV) is one of the major allergens in fish. The aim of our present work was to research the influence mechanism of glycation with different reducing sugars (glucose, fructose, ribose, lactose, and galactose) on the immunoglobulin E (IgE) and immunoglobulin G (IgG) binding capacity and structure changes of PV in Alaska Pollock. PV glycated with glucose or fructose (PV-Glu/ PV-Fru) exhibited the higher IgE/IgG binding capacities than that of ribose, galactose, or lactose. During glycation, the lysine (Lyr), tyrosine (Tyr), and phenylalanine (Phe) of PV were gradually embed into core area of three-dimensional structure of protein, which reflected in the ultraviolet (UV) spectrum and fluorescence spectra. Moreover, the increase of surface hydrophobicity had confirmed the conformation alteration of glycated PV. These results suggest that there is a specific association among the change of PV in glycation and in potential allergenicity. The types and conformation of reducing sugar greatly influenced the IgE/IgG binding capacity of PV, and glycation with ribose and galactose was a promising approach for reducing the IgE/IgG binding capacity of PV from Alaska Pollock.Practical applications Parvalbumin (PV), the major allergen of fish, it can not only maintain the physiological activity of cells, but also cross react with human amyloid protein to alleviate Alzheimer's disease and Parkinson's syndrome. This study revealed that the IgE/IgG binding capacity and structural changes of PV from Alaska Pollock modified by glycation with different reducing sugars. This will help us to understand the sensitization and structural change of the glycated products after the reaction of PV with different reducing sugars. It provides an effective carbonyl source for the preparation of low antigenicity PV based on glycation and lays a foundation for glycation modification of other food allergens.