Mos1-mediated insertional mutagenesis in Caenorhabditis elegans
Mos1-mediated insertional mutagenesis in Caenorhabditis elegans
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DOI：
10.1038/nprot.2007.192
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Bessereau, Jean-Louis
中科院分区：
文献类型：
--
作者：
Boulin, Thomas;Bessereau, Jean-Louis
我们描述了一个协议突变的线虫中的基因使用的Mos1转座子的毛里求斯果蝇。含有Mos 1插入的突变基因被这种异源转座因子分子标记。因此，仅使用基本的分子生物学技术就可以在短短3周内鉴定出Mos1插入。Mos1的诱变效率比经典的化学诱变剂低10倍。然而，与经典遗传图谱绘制中涉及的大量工作相比，诱变插入图谱绘制的容易程度和速度是有利的。因此，当筛选程序有效时，Mos1可能是选择的工具。此外，Mos1诱变可以大大简化表现出低突变率、细微或合成表型的突变的作图。本文综述了近年来C.携带Mos1插入的线虫基因座进一步增加了Mos1介导的诱变的吸引力。
We describe a protocol for mutating genes in the nematode Caenorhabditis elegans using the Mos1 transposon of Drosophila mauritiana. Mutated genes containing a Mos1 insertion are molecularly tagged by this heterologous transposable element. Mos1 insertions can therefore be identified in as little as 3 weeks using only basic molecular biology techniques. Mutagenic efficiency of Mos1 is tenfold lower than classical chemical mutagens. However, the ease and speed with which mutagenic insertions can be mapped compares favorably with the vast amount of work involved in classical genetic mapping. Therefore, Mos1 could be the tool of choice when screening procedures are efficient. In addition, Mos1 mutagenesis can greatly simplify the mapping of mutations that exhibit low penetrance, subtle or synthetic phenotypes. The recent development of targeted engineering of C. elegans loci carrying Mos1 insertions further increases the attractiveness of Mos1-mediated mutagenesis.