Fluorescent Probes for the Detection of Hydrogen Sulfide in Biological Systems
Fluorescent Probes for the Detection of Hydrogen Sulfide in Biological Systems
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DOI：
10.1002/anie.201107025
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发表时间：
2012-01-01
影响因子：
16.6
通讯作者：
Wang, Wei
中科院分区：
文献类型：
--
作者：
Xuan, Weimin;Sheng, Chunquan;Wang, Wei
虽然硫化氢（H2S）被认为是一种有毒气体，具有臭鸡蛋的难闻气味，但这并不能改变它存在于人体和其他生物系统中的事实。据报道，H2S在血液中的典型浓度范围为10-100 μm。[1]显然，它的存在必然与生物学功能有关，例如，它已被认为是除一氧化氮（NO）和一氧化碳（CO）之外的第三种气体信号分子。[2]内源性产生的H2S的重要性已在许多生理和病理过程中得到验证，例如调节细胞生长，[3]心血管保护，[4]刺激血管生成，[5]和抗氧化作用。[6]另一方面，它的失调与阿尔茨海默病，[7]唐氏综合征，[8]糖尿病，[9]和肝硬化的症状有关。[10]由于了解H2S的生物学功能具有巨大的潜在益处，最近对H2S的研究受到了相当大的关注。[11]因此，能够探测H2S在复杂生物系统中的分布和功能的灵敏和选择性检测技术是非常有价值的。目前可用于检测H2S的方法包括比色法，[12]电化学法，[13]和气相色谱法。[14]然而，这些技术不允许反应性和瞬态H2S的时间和空间监测。此外，需要繁琐和复杂的样品制备。虽然荧光
Although hydrogen sulfide (H2S) is known as a toxic gas with the unpleasant smell of rotten eggs, this cannot change the fact that it exists in the human body and other biological systems. It is reported that the typical concentration of H2S in blood is in the range of 10–100 μm.[1] Clearly its presence must be connected with biological functions, for example, it has been recognized as the third gaseous signaling molecule besides nitric oxide (NO) and carbon monoxide (CO).[2] The significance of endogenously produced H2S has been validated in a number of physiological and pathological processes, such as the regulation of cell growth,[3] cardiovascular protection,[4] the stimulation of angiogenesis,[5] and antioxidative effects.[6] On the other hand, its deregulation has been correlated with the symptoms of Alzheimer s disease,[7] Down s syndrome,[8] diabetes,[9] and liver cirrhosis.[10] As a result of the huge potential benefits of understanding the biological functions of H2S, recent research on H2S is gaining considerable attention.[11] Accordingly, sensitive and selective detection techniques that enable the distribution and function of H2S in complicated biological systems to be probed are highly valuable.Current methods that are available for the detection of H2S include colorimetric,[12] electrochemical,[13] and gas chromatography assays.[14] However, these techniques do not allow the temporal and spatial monitoring of reactive and transient H2S. Furthermore, tedious and complicated preparation of the samples is required. Although fluorescent