Role of Ca2+ in phenolic compound metabolism of barley (Hordeum vulgare L.) sprouts under NaCl stress
Role of Ca2+ in phenolic compound metabolism of barley (Hordeum vulgare L.) sprouts under NaCl stress
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氯化钠胁迫下 Ca2+ 在大麦芽酚类化合物代谢中的作用
DOI：
10.1002/jsfa.9764
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发表时间：
2019-08-30
影响因子：
4.1
通讯作者：
Yang, Runqiang
中科院分区：
文献类型：
--
作者：
Ma, Yan;Wang, Pei;Yang, Runqiang
背景钙离子（Ca 2+）在酚类化合物代谢及相关酶活性中的作用的文献仍存在争议。Ca ~（2+）对大麦芽酚类物质含量的影响尚不清楚。本研究以大麦（Hordeum vulgare L.）在氯化钠（NaCl）胁迫下发芽。结果氯化钙（CaCl 2）通过调节酚类化合物合成相关基因、蛋白质表达和酶活性，显著提高总钙、钙调素（CaM）含量和Ca 2+浓度，促进酚类化合物的积累。CaCl 2通过上调苯丙氨酸解氨酶（PAL）、肉桂酸4-羟化酶（C4 H）、4-香豆酸辅酶A连接酶（4CL）和阿魏酸5-羟化酶（F5 H）的蛋白表达，显著提高了它们的活性。种子萌发过程中，对香豆酸3-羟化酶（C3 H）活性降低，咖啡酸O-甲基转移酶（COMT）活性先升高后降低，这与CaCl 2处理下基因和蛋白表达的变化相一致。相反，氯化镧（III）（LaCl 3），乙二醇四乙酸（EGTA）和2-氨基乙氧基二苯基硼酸酯（2-APB）诱导相反的效果。细胞内钙、钙调素（CaM）含量和Ca ~（2+）浓度降低，相关基因和蛋白表达及PAL、C_4H、4CL、C_3H、COMT和F_5H活性的波动变化。结论钙离子在盐胁迫诱导大麦芽酚类物质积累过程中起重要作用。它需要Ca 2+流入和释放质外体和细胞内的商店，分别。(c)2019化学工业协会
BACKGROUND The literature on the role of calcium ion (Ca2+) in relation to phenolic compounds metabolism and related enzymes activities remains controversial. It is still unclear whether Ca2+ affects phenolic compounds content of barley sprouts. This study investigated the role and function of Ca2+ in phenolic compound metabolism of barley (Hordeum vulgare L.) sprouts under sodium chloride (NaCl) stress. RESULTS Calcium chloride (CaCl2) significantly improved total calcium and calmodulin (CaM) contents as well as Ca2+ concentration, and enhanced phenolic compound accumulation by regulating the gene, protein expression and the activities of enzymes related to phenolics biosynthesis. Specifically, CaCl2 significantly increased the activities of phenylalanine ammonia lyase (PAL), cinnamic acid 4-hydroxylase (C4H), 4-coumarate coenzyme A ligase (4CL) and ferulic acid 5-hydroxylase (F5H) by up-regulating the corresponding protein expression. The activity of p-coumaric acid 3-hydroxylase (C3H) decreased during germination while caffeic acid O-methyltransferase (COMT) increased initially and then decreased, which was consistent with the changes in gene and protein expression under CaCl2 treatment. Conversely, lanthanum(III) chloride (LaCl3), ethylene glycol tetraacetic acid (EGTA) and 2-aminoethoxydiphenyl borate (2-APB) induced opposite effects. Decreased calcium and CaM contents and Ca2+ concentration were observed, and fluctuation change of relevant gene and protein expressions and PAL, C4H, 4CL, C3H, COMT and F5H activitives were also detected. CONCLUSION Calcium ion played an important role for mediating NaCl stress-induced phenolics accumulation in barley sprouts. It required both Ca2+ influx and release from apoplast and intracellular stores, respectively. (c) 2019 Society of Chemical Industry