A molecular survey of freshwater microeukaryotes in an Arctic reservoir (Svalbard, 79A°N) in summer by using next-generation sequencing
A molecular survey of freshwater microeukaryotes in an Arctic reservoir (Svalbard, 79A°N) in summer by using next-generation sequencing
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DOI：
10.1007/s00300-014-1576-9
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发表时间：
2015-02-01
期刊：
影响因子：
1.7
通讯作者：
Ki, Jang-Seu
中科院分区：
文献类型：
--
作者：
Boopathi, Thangavelu;Faria, Daphne Georgina;Ki, Jang-Seu
淡水微真核生物的分子多样性，特别是浮游植物，在北极斯瓦尔巴特群岛，一直相对未被探索。淡水藻类被认为是环境变化的生物指标，可用于评估全球气候变化和环境污染增加的影响。在这项研究中，淡水微真核生物在北极水库Ny-C（0）lesund（斯瓦尔巴群岛，挪威）进行了研究，使用高变V1-V3小亚基rRNA和454焦磷酸测序。在8，956个读数的基础上，我们揭示了真核生物中的高遗传多样性，代表了所有已知的真核生物超群，除了挖掘。“Chromalveolata”（以前的超群Chromalveolata）和古质体纲分别是丰度最高和最低的超群。经过数据挖掘，从7,398个热解序列中检测到57个热解类型。其中以沟鞭藻（84%）和沟鞭藻（13%）为主，隐藻门、绿藻门和端丝藻门的贡献较小.属于Mamiellales和Monomastigales的藻类的检测提供了一个窗口，可以看到以前在这种环境中未被检测到的“稀有生物圈”的一部分。有趣的是，没有记录到附着菌。层鞭藻和沟鞭藻主要包括藻纲、合脉藻纲和沟鞭藻纲的类群。根据操作分类单位的比例，发现优势类群包括Ochromonas spp.，毛单胞菌属，和Uroglena americana。这些结果表明，一个金藻为主的microeukaryotic社区的意义，这是未来的研究在重建过去的气候趋势和监测环境变化在北极方面的巨大潜力。
The molecular diversity of freshwater microeukaryotes, particularly phytoplankton, in the Arctic Svalbard, has been relatively unexplored. Freshwater algae are considered biological indicators of environmental change and can be useful in assessing the impact of global climate change and increased environmental pollution. In this study, freshwater microeukaryotes in an Arctic reservoir at Ny-C(0)lesund (Svalbard, Norway) were studied using the hypervariable V1-V3 small subunit rRNA and 454 pyrosequencing. On the basis of 8,956 reads, we revealed high genetic diversity in eukaryotes, representing all known eukaryotic supergroups, except Excavata. "Chromalveolata" (previously supergroup Chromalveolata) and Archaeplastida were the most and least abundant supergroups, respectively. After data mining, 57 phylotypes were detected from 7,398 pyrosequences. They were dominated by stramenopiles (84 %) and Dinoflagellata (13 %), with minor contributions from Cryptophyta, Chlorophyta, and Telonemida. The detection of algae belonging to the orders Mamiellales and Monomastigales provides a window into a fraction of the 'rare biosphere' that had previously been undetected in such environments. Interestingly, no haptophytes were recorded. Stramenopiles and Dinoflagellata mainly comprised taxa belonging to the families Chrysophyceae, Synurophyceae, and Dinophyceae. On the basis of the proportion of operational taxonomic units, the dominant phylotypes were found to include Ochromonas spp., Mallomonas spp., and Uroglena americana. These results demonstrate the significance of a chrysophyte-dominated microeukaryotic community, which is of great potential for future studies in terms of reconstruction of past climate trends and monitoring of environmental change in the Arctic.