Determining the Origin of the Coffee Berry Borer Invasion of Hawaii
Determining the Origin of the Coffee Berry Borer Invasion of Hawaii
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DOI：
10.1093/aesa/sav024
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发表时间：
2015-07-01
影响因子：
2.3
通讯作者：
Harwood, James D.
中科院分区：
文献类型：
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作者：
Chapman, Eric G.;Messing, Russell H.;Harwood, James D.
咖啡浆果蛀虫Hypothenemus hampei（鞘翅目：小蠹科：小蠹亚科）于2010年在夏威夷大岛的咖啡农场首次发现，经过200多年的无蛀虫咖啡生产。因为H.汉贝可能从50多个咖啡生产国进入夏威夷，确定入侵路线需要基因分析。先前的研究确定了27个H。来自世界各地的hampei细胞色素c氧化酶亚基I单倍型使用系统发育分析，以确定推定的物种。我们对采集于夏威夷的标本进行了细胞色素c氧化酶亚基I的测序，并进行了系统发育和单倍型网络分析，以追踪入侵途径。我们进行了一个网络分析，以跟踪最有可能的途径，H。hampei可能已经采取了夏威夷和系统发育分析，以评估分支支持更广泛的群体在网络分析，不太可能最近杂交。夏威夷的单倍型与六个拉丁美洲国家的单倍型相同，我们的网络分析表明，最可能的入侵路线是从肯尼亚到乌干达，再到拉丁美洲，再到夏威夷。大多数来自拉丁美洲的咖啡都经过熏蒸，到达奥克拉荷马岛，并在运往其他岛屿之前进行加工。因此，H.虽然来自拉丁美洲的货物仍有可能被不适当的熏蒸处理，但来自拉丁美洲的农场工人或其他旅行者在衣服或行李中携带了受钻孔虫感染的种子，或者少量非法进口的豆类，意外地将hampei运到了大岛。
The coffee berry borer, Hypothenemus hampei (Coleoptera: Curculionidae: Scolytinae), was first discovered in coffee farms on the Big Island of Hawaii in 2010, after over 200 yr of borer-free coffee production. Because there are multiple pathways by which H. hampei could have entered Hawaii from >50 coffee-producing nations that harbor the pest, determining the invasion route requires genetic analyses. A previous study identified 27 H. hampei cytochrome c oxidase subunit I haplotypes from around the world using phylogenetic analyses to identify putative species. We sequenced cytochrome c oxidase subunit I from specimens collected in Hawaii and conducted phylogenetic and haplotype network analyses to trace the route of invasion. We conducted a network analysis to trace the most likely pathway that H. hampei could have taken to Hawaii and a phylogenetic analysis to assess clade support for broader groupings in the network analysis that are unlikely to have recently hybridized. The Hawaiian haplotype was identical to a haplotype from six Latin American countries, and our network analysis suggests the most likely route of invasion was from Kenya to Uganda to Latin America to Hawaii. Most coffee shipments from Latin America are fumigated, arrive on Oahu, and are processed before being shipped to other islands. Therefore, it is likely that H. hampei was accidentally transported to the Big Island by farm workers or other travelers from Latin America who carried borer-infested seeds in their clothing or luggage, or else by small quantities of illegally imported beans, although improper fumigation of shipments from Latin America remains a possibility.