Monitoring human enteric viruses in wastewater and relevance to infections encountered in the clinical setting: a one-year experiment in central France, 2014 to 2015.
Monitoring human enteric viruses in wastewater and relevance to infections encountered in the clinical setting: a one-year experiment in central France, 2014 to 2015.
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DOI：
10.2807/1560-7917.es.2018.23.7.17-00237
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发表时间：
2018-03
期刊：
影响因子：
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通讯作者：
Henquell C
中科院分区：
文献类型：
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作者：
Bisseux M;Colombet J;Mirand A;Roque-Afonso AM;Abravanel F;Izopet J;Archimbaud C;Peigue-Lafeuille H;Debroas D;Bailly JL;Henquell C
人类肠道病毒在环境中具有耐药性，并通过粪-口途径传播。废水中的病毒脱落为追踪新出现的病原体和研究社区肠道传染病的流行病学提供了机会。目的：本研究的目的是通过分析城市废水来监测克莱蒙-费兰德地区（法国）人群中肠道病毒的传播。研究方法：在2014年10月至2015年10月期间收集了未经处理的废水和经处理的废水，并使用切向流超滤和聚乙二醇沉淀通过两步方案进行浓缩。对处理后的样本进行了腺病毒、诺如病毒、轮状病毒、双埃柯病毒、肠道病毒（EV）、甲型肝炎（HAV）和戊型肝炎（HEV）病毒的分子检测分析。结果：所有废水样品（n = 54）含有病毒。平均而言，分别在未经处理和经处理的废水样品中检测到六种和四种病毒。对EV阳性样本进行EV-D 68检测，以评估其在社区中的传播情况。在27份原始样品中的7份中检测到EV-D 68。我们收集了EV-D 68（n = 17），HAV（n = 4）和HEV感染（n = 16）的临床病例的数据，并将废水来源的序列与临床序列进行了比较。我们在2015年9月展示了EV-D 68的沉默循环，尽管很少有急性疾病的通知，但HAV的广泛循环以及在废水中存在涉及临床本地病例的主要HEV亚型。结论：环境监测克服了与住院相关的感染研究固有的采样偏倚，并允许在真实的时间内检测与临床标本中报告的病毒序列在遗传上接近的病毒序列。
Human enteric viruses are resistant in the environment and transmitted via the faecal-oral route. Viral shedding in wastewater gives the opportunity to track emerging pathogens and study the epidemiology of enteric infectious diseases in the community. Aim: The aim of this study was to monitor the circulation of enteric viruses in the population of the Clermont-Ferrand area (France) by analysis of urban wastewaters. Methods: Raw and treated wastewaters were collected between October 2014 and October 2015 and concentrated by a two-step protocol using tangential flow ultrafiltration and polyethylene glycol precipitation. Processed samples were analysed for molecular detection of adenovirus, norovirus, rotavirus, parechovirus, enterovirus (EV), hepatitis A (HAV) and E (HEV) viruses. Results: All wastewater samples (n = 54) contained viruses. On average, six and four virus species were detected in, respectively, raw and treated wastewater samples. EV-positive samples were tested for EV-D68 to assess its circulation in the community. EV-D68 was detected in seven of 27 raw samples. We collected data from clinical cases of EV-D68 (n = 17), HAV (n = 4) and HEV infection (n = 16) and compared wastewater-derived sequences with clinical sequences. We showed the silent circulation of EV-D68 in September 2015, the wide circulation of HAV despite few notifications of acute disease and the presence in wastewater of the major HEV subtypes involved in clinical local cases. Conclusion: The environmental surveillance overcomes the sampling bias intrinsic to the study of infections associated with hospitalisation and allows the detection in real time of viral sequences genetically close to those reported in clinical specimens.