Profiling the oral microbiomes in patients with Alzheimer's disease
Profiling the oral microbiomes in patients with Alzheimer's disease
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分析阿尔茨海默病患者的口腔微生物组
DOI：
10.1111/odi.14110
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发表时间：
2022-01-04
期刊：
影响因子：
3.8
通讯作者：
Du, Minquan
中科院分区：
文献类型：
--
作者：
Guo, Haiying;Li, Biao;Du, Minquan
目的分析口腔微生物组的特征，寻找阿尔茨海默病（AD）的生物标志物。研究对象与方法对26例AD患者和26例认知完好者进行认知、抑郁、口腔健康检查，并于早晨采集唾液和龈沟液（GCF）。扩增16S rRNA全长基因，采用PacBio平台进行测序。结果AD患者唾液菌群优势菌群为口腔链球菌，牙周菌群优势菌群为牙龈卟啉单胞菌。在β多样性分析方面，AD患者与认知完好的受试者牙周微生物组存在显著差异。阿尔茨海默病患者口腔微生物组中细小细孔菌的相对丰度显著增加。有趣的是，优势种在早发性AD和晚发性AD患者之间存在差异。此外，优势种随阿尔茨海默病的临床严重程度而变化。此外，相关分析显示，细小弧菌在唾液和龈沟中都与AD相关，而牙龈弧菌仅在龈沟中与AD相关。结论AD患者口腔微生物群落发生改变，牙周微生物群落对认知变化较为敏感。此外，细小弧菌和牙龈弧菌与AD相关。
Objectives To analyse the characteristics of the oral microbiomes and expected to find biomarkers about Alzheimer's disease (AD). Subjects and Methods AD patients (n = 26) and cognitive intact people (n = 26) were examined for cognition, depression, oral health and collected saliva and gingival crevicular fluid (GCF) in the morning. Full-length 16S rRNA gene was amplified and sequencing was performed using the PacBio platform. Results The predominant bacterium of salivary microbiome and periodontal microbiome from AD patients was Streptococcus oralis and Porphyromonas gingivalis, respectively. With respect to beta diversity analysis, there was a significance difference in periodontal microbiome between AD patients and cognitively intact subjects. The relative abundance of Veillonella parvula significantly increased in oral microbiomes from AD patients. Interestingly, the dominant species were different between early-onset AD and late-onset AD patients. Moreover, the predominant species were changed as the clinical severity of AD. Furthermore, the correlation analysis revealed that V. parvula was associated with AD in both saliva and GCF and that P. gingivalis was associated with AD only in GCF. Conclusions In this study, the microbiome community of oral microbes was altered in AD patients and periodontal microbiome was sensitive to cognition changes. Moreover, V. parvula and P. gingivalis were associated with AD.