Upregulation of M-creatine kinase and glyceraldehyde-3-phosphate dehydrogenase:: two markers of muscle disuse
Upregulation of M-creatine kinase and glyceraldehyde-3-phosphate dehydrogenase:: two markers of muscle disuse
复制标题
DOI：
10.1152/ajpregu.1999.276.2.r308
复制
发表时间：
1999-02-01
影响因子：
2.8
通讯作者：
Dechesne, CA
中科院分区：
文献类型：
--
作者：
Cros, N;Muller, J;Dechesne, CA
肌肉停用会导致高度可塑性的骨骼肌组织发生实质性的变化，这种变化尤其发生在反重力缓慢的肌肉中。我们差异化地筛选了一个肌肉基因表达谱阵列，以确定通过后肢悬吊机械卸载的慢速大鼠比目鱼肌的基因表达变化，作为肌肉废用的模型。本研究的重点是肌肉肌酸激酶基因和蛋白以及甘油醛-3-磷酸脱氢酶基因的表达，这两种基因在未称重的肌肉中表达上调。这些上调是在后肢悬吊4周的时间过程中分析的，并与肌球蛋白重链(MHC)亚型的变化进行比较，同时特别关注IIx型MHC的mRNA和蛋白。这两个代谢标志物的上调明显先于IIx MHC收缩蛋白的上调。肌肉肌酸激酶的上调被证明是一个很好的，也是最早的，在mRNA和蛋白质水平上肌肉停用的标志。
Muscle disuse induces substantial alterations in the highly plastic skeletal muscle tissues, which occur especially in antigravity slow muscles. We differentially screened a muscle cDNA array to identify modifications in gene profile expression induced in slow rat soleus muscle mechanically unloaded by hindlimb suspension as a model for muscle disuse. This study focused on muscle creatine kinase mRNA and protein and glyceraldehyde-3-phosphate dehydrogenase mRNA, which were found to be upregulated in unweighted muscles. These upregulations were analyzed over a 4-wk time course of hindlimb suspension and compared with variations in myosin heavy chain (MHC) isoforms while specifically focusing on type IIx MHC mRNA and protein. The two metabolic marker upregulations clearly preceded IIx MHC contractile protein upregulation. Muscle creatine kinase upregulation was shown to be an excellent, and the earliest, marker of muscle disuse at mRNA and protein levels.