Ascorbate prevents prooxidant effects of urate in oxidation of human low density lipoprotein
Ascorbate prevents prooxidant effects of urate in oxidation of human low density lipoprotein
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DOI：
10.1016/s0014-5793(99)00231-8
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发表时间：
1999-03-12
期刊：
影响因子：
3.5
通讯作者：
Abuja, PM
中科院分区：
文献类型：
--
作者：
Abuja, PM
尿酸和抗坏血酸是血浆中重要的低分子量抗氧化剂，本文研究了它们的相互作用及联合作用对Cu ~（2+）催化人低密度脂蛋白氧化的影响。已经发现，只要在氧化开始后不久将尿酸加入到低密度脂蛋白中，尿酸单独就成为强促氧化剂（条件促氧化剂）。抗坏血酸在人血浆中的浓度（20-60 μ M）比尿酸盐（300-400 μ M）低得多，它本身不是条件促氧化剂。此外，当与尿酸盐同时添加到氧化低密度脂蛋白中时，抗坏血酸盐阻止尿酸盐的促氧化作用，即使尿酸盐比抗坏血酸盐摩尔过量60倍。抗坏血酸盐似乎具有与其它水性还原剂相同的抗促氧化作用，所述其它水性还原剂除了它们的抗氧化性质之外，仅报道为条件性促氧化剂，抗坏血酸盐和尿酸盐之间的这种相互作用在防止循环和其他生物流体中脂蛋白的氧化修饰中可能是重要的，（C）1999欧洲生物化学学会联合会。
Uric acid and ascorbic acid are important low molecular weight antioxidants in plasma, Their interactions and combined effect on Cu2+-catalysed oxidation of human low density lipoprotein mere studied in vitro. It was found that uric acid alone becomes strongly prooxidant whenever it is added to low density lipoprotein shortly after the start of oxidation (conditional prooxidant), Ascorbic acid, which is present in human plasma at much lower concentrations (20-60 mu M) than urate (300-400 mu M), is in itself not a conditional prooxidant. Moreover, ascorbate prevents prooxidant effects of urate, when added to oxidising low density lipoprotein simultaneously with urate, even at a 60-fold molar excess of urate over ascorbate, Ascorbate appears to have the same anti-prooxidant effect with other aqueous reductants, which, besides their antioxidant properties, mere reported to be conditionally prooxidant, Such interactions between ascorbate and urate may be important in preventing oxidative modification of lipoproteins in the circulation and in other biological fluids, (C) 1999 Federation of European Biochemical Societies.