Temperature tolerances of wild-type and red transgenic zebra danios
Temperature tolerances of wild-type and red transgenic zebra danios
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DOI：
10.1577/t04-197.1
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发表时间：
2005-11-01
影响因子：
1.4
通讯作者：
Beitinger, TL
中科院分区：
文献类型：
--
作者：
Cortemeglia, C;Beitinger, TL
79只转基因红斑马（也称为斑马鱼;在20 ℃和30 ℃下驯化的78只野生型斑马鱼和78只转基因斑马鱼的临界最大温度（+/- SD）分别为38.8 +/- 0.54 ℃和41.3 +/- 0.50 ℃（glofish）和39.2 +/- 0.34 ℃和41.7 +/-0.50 ℃（glofish）。-0.35 ° C（野生型）。临界热最小值（+/- SD）分别为7.2 +/- 0.48 ℃和10.0 +/- 0.50 ℃（河豚）和6.2 +/- 0.28 ℃和10.6 +/- 0.53 ℃（野生型）。在20摄氏度和30摄氏度的驯化温度下，转基因斑马鱼和野生斑马鱼的临界最大温度（CTMax）和临界最小温度（CTMin）之间存在显著差异;然而，差异为1摄氏度或更小。温度耐受性将两种斑马鱼归类为广温性，并将该物种置于相对罕见的鱼类类别中，CTMax高于40.0摄氏度。与已成功引入美国沃茨的其他热带鱼报道的类似温度耐受性数据相比，目前的数据表明野生型斑马鱼和红河豚都能够在美国南部的一些沃茨越冬。
Seventy-nine transgenic red zebra danios Danio rerio (also known as zebrafish; transgenics also known as glofish) and 78 wild-type zebra danios acclimated to 20 C and 30 C had critical thermal maxima (+/- SD) of 38.8 +/- 0.54 degrees C and 41.3 +/- 0.50 degrees C (glofish) and 39.2 +/- 0.34 degrees C and 41.7 +/- 0.35 degrees C (wild-type), respectively. The critical thermal minima (+/- SD) were 7.2 +/- 0.48 C and 10.0 +/- 0.50 degrees C (glofish) and 6.2 +/- 0.28 degrees C and 10.6 +/- 0.53 degrees C (wild-type), respectively. At both 20 degrees C and 30 degrees C acclimation temperatures, critical thermal maxima (CTMax) and critical thermal minima (CTMin) were significantly different between transgenic and wild zebrafish; however, differences were 1 C and less. The temperature tolerances would classify both varieties of zebra danios as eurythermal and place this species in the relatively rare category of fishes with a CTMax above 40.0 degrees C. Compared with similar temperature tolerance data reported for other tropical fishes that have successfully been introduced into U.S. waters, the present data suggest both wildtype zebra danios and red glofish are capable of overwintering in some southern U.S. waters.