TRANSFORMING GROWTH-FACTOR-BETA-1 HAS BOTH PROMOTING AND INHIBITING EFFECTS ON INDUCTION OF DNA-SYNTHESIS IN HUMAN FIBROBLASTS
TRANSFORMING GROWTH-FACTOR-BETA-1 HAS BOTH PROMOTING AND INHIBITING EFFECTS ON INDUCTION OF DNA-SYNTHESIS IN HUMAN FIBROBLASTS
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DOI：
10.1006/excr.1995.1014
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发表时间：
1995-01-01
影响因子：
3.7
通讯作者：
IDE, T
中科院分区：
文献类型：
--
作者：
FUKAMI, J;TSUJI, K;IDE, T
生长停滞的人类正常成纤维细胞 TIG-1 在添加表皮生长因子 (EGF) 后启动 DNA 合成。转化生长因子-β 1 (TGF-β 1) 本身对 DNA 合成的诱导没有影响。当EGF和TGF-β1同时添加到生长停滞的TIG-1细胞中时，与单独使用EGF相比，DNA合成的诱导增强。相反，当TGF-β1的添加早于EGF添加2小时或晚于2小时时，DNA合成的诱导被阻止。EGF诱导的DNA合成对百日咳毒素（PT，Gi蛋白抑制剂）和十字孢菌素（蛋白激酶抑制剂）不敏感。 TGF-β 1 对 DNA 合成的促进作用是 PT 不敏感而星形孢菌素敏感，相反，TGF-β 1 对 DNA 合成的抑制活性是 PT 敏感而星形孢菌素不敏感。这些研究表明，TGF-β 1 的作用是促进或抑制同一细胞中通过不同信号转导过程表达的 EGF 对 DNA 合成的诱导。 (C) 1995 学术出版社
Growth-arrested human normal fibroblasts, TIG-1, initiated DNA synthesis following addition of epidermal growth factor (EGF). Transforming growth factor-beta 1 (TGF-beta 1) by itself had no effect on induction of DNA synthesis. When EGF and TGF-beta 1 were added simultaneously to growth-arrested TIG-1 cells, induction of DNA synthesis was enhanced compared with that by EGF alone. Contrarily, when TGF-beta 1 was added earlier than 2 h or later than 2 h of EGF addition, induction of DNA synthesis was prevented, Induction of DNA synthesis by EGF was insensitive to pertusis toxin (PT, an inhibitor of Gi protein) and to staurosporine (a protein kinase inhibitor). The promoting effect of TGF-beta 1 on DNA synthesis was PT-insensitive and staurosporine-sensitive, Contrarily, inhibitory activity of TGF-beta 1 on DNA synthesis was PT-sensitive and staurosporine-insensitive, These studies suggest that the effect of TGF-beta 1 is to promote or to inhibit induction of DNA synthesis by EGF expressed through different signal transduction processes in the same cell. (C) 1995 Academic Press, Inc.