NEW SHUTTLE VECTOR FOR CLONING IN BACILLUS-STEAROTHERMOPHILUS
NEW SHUTTLE VECTOR FOR CLONING IN BACILLUS-STEAROTHERMOPHILUS
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DOI：
10.1016/0923-2508(94)90074-4
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发表时间：
1994-10-01
影响因子：
2.6
通讯作者：
MATTEUZZI, D
中科院分区：
文献类型：
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作者：
DEROSSI, E;BRIGIDI, P;MATTEUZZI, D
克隆载体质粒pRP9是在广宿主范围质粒pLM6的基础上构建的。 pRP9 是一个小质粒（2.9 kb），具有方便的多聚限制性位点序列，可有效转化枯草芽孢杆菌、嗜热脂肪芽孢杆菌和大肠杆菌。此外，pRP9 在芽孢杆菌宿主中表现出非常高的分离稳定性。而且，在选择压力下生长的芽孢杆菌菌株中，携带 3-kb 片段的 pRP9 的结构稳定性很高。在嗜热脂肪芽孢杆菌中未发现单链高分子量 pRP9 DNA。所描述的宿主/载体系统具有高效基因克隆所需的所有特性。
Cloning vector plasmid pRP9 was constructed on the basis of the broad host-range plasmid pLM6. pRP9 was a small plasmid (2.9 kb), possessed a convenient polyrestriction site sequence and efficiently transformed Bacillus subtilis, Bacillus stearothermophilus and Escherichia coli. Furthermore, pRP9 presented a very high segregational stability in Bacillus hosts. Also, the structural stability in Bacillus strains, grown under selective pressure, of pRP9 carrying a 3-kb fragment, was high. No single-stranded and high-molecular weight pRP9 DNA was found in B. stearothermophilus. The host/vector systems described possessed all the properties required far efficient gene cloning.