HEAT-RESISTANCE OF BACTERIAL-SPORES CORRELATED WITH PROTOPLAST DEHYDRATION, MINERALIZATION, AND THERMAL ADAPTATION
HEAT-RESISTANCE OF BACTERIAL-SPORES CORRELATED WITH PROTOPLAST DEHYDRATION, MINERALIZATION, AND THERMAL ADAPTATION
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DOI：
10.1128/aem.52.6.1242-1246.1986
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发表时间：
1986-12-01
影响因子：
4.4
通讯作者：
GERHARDT, P
中科院分区：
文献类型：
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作者：
BEAMAN, TC;GERHARDT, P
在代表嗜热菌、嗜温菌和嗜冷菌的 7 种芽孢杆菌中获得了 28 种溶菌酶敏感孢子，其耐湿热性跨越了 3,000 倍的范围。物种内的抗性通过天然孢子对质子化孢子的脱矿质和质子化孢子对钙化孢子的再矿化以及通过在最高、最佳和最低孢子形成温度下的热适应而改变。原生质体湿密度以及原生质体水含量通过 Nycodenz 梯度中的浮力密度沉降获得（Nyegaard and Co.，Oslo，Norway）。矿化和热适应的增加导致原生质体含水量减少到大约极限值之间。 57% 和 28%（湿重基础），因此与孢子耐热性的增加相关。然而，高于和低于这些限制，矿化和热适应的增加与孢子抵抗力的增加相关，而与原生质体水含量不变无关。所有这三个因素显然都有助于孢子的耐热性，并且是孢子耐热性所必需的，但脱水占主导地位。
Twenty-eight types of lysozyme-sensitive spores among seven Bacillus species representative of thermophiles, mesophiles, and psychrophiles were obtained spanning a 3,000-fold range in moist-heat resistance. The resistance within species was altered by demineralization of the native spores to protonated spores and remineralization of the protonated spores to calcified spores and by thermal adaptation at maximum, optimum, and minimum sporulation temperatures. Protoplast wet densities, and thereby protoplast water contents, were obtained by buoyant density sedimentation in Nycodenz gradients (Nyegaard and Co., Oslo, Norway). Increases in mineralization and thermal adaptation caused reductions in protoplast water content between limits of ca. 57 and 28% (wet weight basis), and thereby correlated with increases in sporal heat resistance. Above and below these limits, however, increases in mineralization and thermal adaptation correlated with increases in sporal resistance independently of unchanged protoplast water contents. All three factors evidently contributed to and were necessary for heat resistance of the spores, but dehydration predominated.