STUDIES ON THE NUTRIENT-UPTAKE BY THE BEET CYST-NEMATODE HETERODERA-SCHACHTII BY IN-SITU MICROINJECTION OF FLUORESCENT-PROBES INTO THE FEEDING STRUCTURES IN ARABIDOPSIS-THALIANA
STUDIES ON THE NUTRIENT-UPTAKE BY THE BEET CYST-NEMATODE HETERODERA-SCHACHTII BY IN-SITU MICROINJECTION OF FLUORESCENT-PROBES INTO THE FEEDING STRUCTURES IN ARABIDOPSIS-THALIANA
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DOI：
10.1017/s003118200007637x
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发表时间：
1994-08-01
期刊：
影响因子：
2.4
通讯作者：
GRUNDLER, FMW
中科院分区：
文献类型：
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作者：
BOCKENHOFF, A;GRUNDLER, FMW
研究了一种将物质注入拟南芥根中沙氏异源菌（Heterodera schachtii）建立的取食结构（合胞体）的方法。该技术用于研究取食线虫对养分的吸收。将荧光染料路西弗黄CH （LYCH）和不同分子量的荧光标记右旋糖酐注射到拟南芥（a.thaliana）薄而半透明的根中。这种根是这种植物的一个特征，它们为显微注射提供了最佳条件。通过消化道和消化系统的染色可以看出，喂食幼鱼和成鱼都摄取了LYCH。摄入的荧光右旋糖聚糖为3、10和20 kDa，而不是40和70 kDa，这表明最大斯托克斯半径为3.2至4.4 nm的分子被吸收。很可能是形成植物细胞质和线虫消化系统之间的界面的喂食管造成了这种大小排斥效应。注射的荧光物质未在合胞体附近的植物细胞或根维管分子中检测到。对被数种线虫侵染的根部进行注射，结果表明，进食的沙克蒂嗜血杆菌可能共用一个合胞体。
A method was developed, which enables substances to be injected into the feeding structure (syncytium) established by Heterodera schachtii in roots of Arabidopsis thaliana. The technique was used to study the uptake of nutrients by the feeding nematode. The fluorescent dye lucifer yellow CH (LYCH) and fluorescence-labelled dextrans of different molecular weights were injected into the thin and translucent roots of A. thaliana. Such roots are a feature of this plant and they provide optimal conditions for microinjection. Injected LYCH was taken up by feeding juveniles and adults, indicated by the staining of the alimentary duct and the digestive system. Fluorescent dextrans of 3, 10 and 20 kDa but not of 40 and 70 kDa were ingested, suggesting that molecules of a maximum Stokes radius of 3.2 to 4.4 nm are taken up. It is likely that the feeding tube, forming the interface between the plant cytosol and the nematode's digestive system, is responsible for this size exclusion effect. The injected fluorescent substances were not detected in plant cells adjacent to the syncytium or in the root vascular elements. Injections into parts of roots which were infested by several nematodes revealed that feeding H. schachtii individuals may share one syncytium.