A90720A, a serine protease inhibitor isolated from a terrestrial blue-green alga Microchaete loktakensis
A90720A, a serine protease inhibitor isolated from a terrestrial blue-green alga Microchaete loktakensis
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DOI：
10.1016/0040-4020(95)00857-8
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发表时间：
1996-01-08
期刊：
影响因子：
2.1
通讯作者：
Patterson, GML
中科院分区：
文献类型：
--
作者：
Bonjouklian, R;Smitka, TA;Patterson, GML
描述了 A90720A,1（一种来自陆生蓝绿藻 Microchaete loktakensis 的环状缩酚酸肽）的分离和结构解析。根据其凝血酶抑制活性分离出1，并使用光谱方法和氨基酸分析确定了其结构。发现组成氨基酸的绝对构型如下：核含有L-苏氨酸、L-精氨酸、L-3-氨基-6-羟基-2-哌啶酮（Ahp，即谷氨酸-γ-甲醛）、L-亮氨酸、L-N-甲基-酪氨酸和L-缬氨酸。 L-苏氨酸氨基的侧链含有 D-亮氨酸，随后是 3-O-硫酸化 R-甘油酸酯部分。
The isolation and structure elucidation of A90720A,1, a cyclic depsipeptide from the terrestrial blue-green alga Microchaete loktakensis is described. 1 was isolated on the basis of its thrombin inhibitory activity and its structure was determined using spectral methods and amino acid analysis. The absolute configurations of the constituent amino acids were found to be as follows: the nucleus contains L-threonine, L-arginine, L-3-amino-6-hydroxy-2-piperidone (Ahp, i.e., glutamic acid-gamma-carboxaldehyde), L-leucine, L-N-methyl-tyrosine and L-valine. The side chain off the L-threonine amino group contains D-leucine, followed by a 3-O-sulfated R-glycerate moiety.